Detailed information of TA system TA034023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 135096..136006
• Replicon: plasmid pWP3-W18-ESBL-06_1
• Accession: NZ_AP021984
• Organism: Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: H7R89_RS27450
• Protein ID: WP_004144067.1
• Coordinates: 135096..135566 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: H7R89_RS27455
• Protein ID: WP_182952499.1
• Coordinates: 135563..136006 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R89_RS27420	130477..130644	+	168	WP_004117900.1	hypothetical protein	-
H7R89_RS27425	130674..130916	+	243	WP_086528906.1	hypothetical protein	-
H7R89_RS27430	131295..131453	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
H7R89_RS27435	131525..132637	+	1113	WP_182952498.1	IS481-like element ISKpn28 family transposase	-
H7R89_RS27445	134725..134985	+	261	WP_004026352.1	hypothetical protein	-
H7R89_RS27450	135096..135566	-	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
H7R89_RS27455	135563..136006	-	444	WP_182952499.1	DUF2384 domain-containing protein	Antitoxin
H7R89_RS27460	136107..137378	-	1272	WP_099745241.1	translesion error-prone DNA polymerase V subunit UmuC	-
H7R89_RS27465	137378..137800	-	423	WP_004197884.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
H7R89_RS27470	137868..139088	-	1221	WP_004194725.1	ISL3-like element ISCfr12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..251197	251197
	-	inside	IScluster/Tn	-	-	126391..143038	16647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T34023 WP_004144067.1 NZ_AP021984:c135566-135096 [Raoultella ornithinolytica]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T34023 NZ_AP021984:c135566-135096 [Raoultella ornithinolytica]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16470.76 Da
• Isoelectric Point: 10.2498

>AT34023 WP_182952499.1 NZ_AP021984:c136006-135563 [Raoultella ornithinolytica]
MKTFSLSSTSARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT34023 NZ_AP021984:c136006-135563 [Raoultella ornithinolytica]
ATGAAAACATTTAGTTTGTCCTCAACTTCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTAAGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A384IED8