Detailed information of TA system TA034022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 45920..46671
• Replicon: plasmid pWP3-W18-ESBL-06_1
• Accession: NZ_AP021984
• Organism: Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: H7R89_RS27005
• Protein ID: WP_101826042.1
• Coordinates: 46189..46671 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A6S4YWG5
• Locus tag: H7R89_RS27000
• Protein ID: WP_012540170.1
• Coordinates: 45920..46198 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R89_RS26975	41047..42051	+	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
H7R89_RS26980	42486..43085	+	600	WP_123282530.1	recombinase family protein	-
H7R89_RS26985	43092..43514	+	423	WP_182952522.1	hypothetical protein	-
H7R89_RS26990	43758..44072	-	315	WP_017146616.1	hypothetical protein	-
H7R89_RS26995	45076..45612	-	537	WP_182952510.1	hypothetical protein	-
H7R89_RS27000	45920..46198	+	279	WP_012540170.1	DUF1778 domain-containing protein	Antitoxin
H7R89_RS27005	46189..46671	+	483	WP_101826042.1	GNAT family N-acetyltransferase	Toxin
H7R89_RS27010	46709..47113	+	405	WP_048293664.1	DUF2251 domain-containing protein	-
H7R89_RS27015	47450..48283	+	834	WP_012540133.1	GIY-YIG nuclease family protein	-
H7R89_RS27020	48347..48544	+	198	WP_109037821.1	bacteriocin immunity protein	-
H7R89_RS27025	48639..48875	-	237	WP_012540047.1	hypothetical protein	-
H7R89_RS27030	49920..50648	+	729	WP_182952511.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..251197	251197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17698.59 Da
• Isoelectric Point: 9.1776

>T34022 WP_101826042.1 NZ_AP021984:46189-46671 [Raoultella ornithinolytica]
MGMRPPEPLTPEHNIADFCCQDQVLSEWLKKKALKNHSTGLSRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTEHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Nucleotide

• Length: 483 bp

>T34022 NZ_AP021984:46189-46671 [Raoultella ornithinolytica]
ATGGGAATGAGGCCTCCGGAACCTCTTACCCCTGAACATAACATTGCTGATTTTTGTTGTCAGGATCAGGTTCTTTCTGA
GTGGTTGAAGAAAAAGGCGCTGAAGAACCATAGCACGGGCCTTTCAAGAGTTTTCGTCGTCTGTGCTGAAAATACGAATA
GGGTAATTGCGTACTACTGTTTGGCATCCGGAAGCGTGCACAGAAACACCGTACCCGGAGCATACCGTCGTAATGCACCA
GAGGCTTTACCGGTCATTGTTCTGGGAAGGCTTGCGGTTGACGCTGCCTGGGCAAGAAAAGGGCTGGGAGCTGCACTGCT
TAAGGATGCGATATACCGAACAGAACATATTGCAATACAGGTTGGTGTTAGGGCGCTTCTTGTTCACGCCTTAAATGATG
AAGTTAGGGAATTTTACACAAAGTTTGGATTCGAGCCCTCCATTGCCAATGCGCTGACATTATTATTTCCGATCAAAACT
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10749.33 Da
• Isoelectric Point: 4.6722

>AT34022 WP_012540170.1 NZ_AP021984:45920-46198 [Raoultella ornithinolytica]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFNAFVEELDAPMTPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT34022 NZ_AP021984:45920-46198 [Raoultella ornithinolytica]
ATGAAACCAGACATTCGTGAGGCTCCGATAAACATCCGGGCGAAAACATCTCAACGCGATCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTGGAAGTGGCCTGCCGCGAAGCGCAGGACATCTTGTTAGATCAGCGCT
TGTTTCTGCTGGATGATAAGCAGTTTAACGCGTTTGTCGAAGAGCTTGACGCCCCTATGACCCCAGAGCGTCAGGCGCGT
ATTGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6S4YWG5