Detailed information of TA system TA034021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 4873..5407
• Replicon: plasmid pWP3-W18-ESBL-06_1
• Accession: NZ_AP021984
• Organism: Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H7R89_RS26770
• Protein ID: WP_123282529.1
• Coordinates: 4873..5157 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: H7R89_RS26775
• Protein ID: WP_123282528.1
• Coordinates: 5144..5407 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R89_RS26760	101..1036	+	936	WP_182952509.1	omptin family outer membrane protease Kop	-
H7R89_RS26765	1706..4786	-	3081	WP_182928827.1	Tn3 family transposase	-
H7R89_RS26770	4873..5157	-	285	WP_123282529.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H7R89_RS26775	5144..5407	-	264	WP_123282528.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H7R89_RS26780	5585..5833	+	249	WP_012585400.1	type II toxin-antitoxin system YafQ family toxin	-
H7R89_RS26785	5960..6535	+	576	WP_001173919.1	recombinase family protein	-
H7R89_RS26790	6660..7262	+	603	WP_010465829.1	recombinase family protein	-
H7R89_RS26795	7246..10275	+	3030	WP_024015041.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..251197	251197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-70)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10843.47 Da
• Isoelectric Point: 8.0737

>T34021 WP_123282529.1 NZ_AP021984:c5157-4873 [Raoultella ornithinolytica]
MRTIEYTSQFKRDFKRESKGQYKAVLAGAEFSLIVQVLANDLPLPEKLRDHPLSGDWKDHRDCHVKPDLVLIYRKPDDTV
LQLVRLGSHSELGL

Nucleotide

• Length: 285 bp

>T34021 NZ_AP021984:c5157-4873 [Raoultella ornithinolytica]
ATGCGGACGATTGAATACACAAGCCAGTTCAAGCGAGATTTTAAACGCGAAAGCAAAGGGCAATACAAAGCGGTTCTTGC
CGGTGCCGAGTTTTCGTTGATAGTTCAAGTTTTGGCAAATGATTTGCCATTGCCTGAAAAACTACGCGATCACCCGCTCT
CAGGTGACTGGAAAGATCATCGGGACTGTCATGTTAAGCCCGATCTGGTTTTAATTTACCGAAAACCAGATGACACCGTA
TTGCAGCTTGTTCGTTTAGGGTCACATAGCGAATTAGGGTTATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9487.95 Da
• Isoelectric Point: 4.6286

>AT34021 WP_123282528.1 NZ_AP021984:c5407-5144 [Raoultella ornithinolytica]
MSANAVVRARIDEHIKEEATIVLASMGLTVSDAFRIMLTRVAREKALPFEPLVPNAETIEAMKEARKGGLKSFATVDDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT34021 NZ_AP021984:c5407-5144 [Raoultella ornithinolytica]
ATGTCTGCAAATGCCGTTGTACGTGCCCGTATTGATGAACACATCAAAGAAGAAGCTACTATCGTTCTGGCATCGATGGG
GCTGACTGTATCTGATGCTTTCCGGATCATGCTGACTCGTGTTGCCCGTGAAAAAGCATTGCCATTCGAGCCATTGGTAC
CCAATGCTGAAACTATTGAAGCTATGAAAGAAGCTCGTAAAGGTGGGTTGAAATCTTTCGCAACAGTTGATGATTTAATG
GCTGATCTCAATGCGGACGATTGA