Detailed information of TA system TA034020

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5033501..5034181
• Replicon: chromosome
• Accession: NZ_AP021983
• Organism: Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: H7R89_RS23995
• Protein ID: WP_182952493.1
• Coordinates: 5033501..5033815 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: H7R89_RS24000
• Protein ID: WP_182952071.1
• Coordinates: 5033858..5034181 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R89_RS23965	5028814..5029335	-	522	WP_069476288.1	hypothetical protein	-
H7R89_RS23970	5029316..5029831	-	516	WP_097358169.1	DUF2778 domain-containing protein	-
H7R89_RS23975	5030270..5030967	+	698	Protein_4703	IS1 family transposase	-
H7R89_RS23980	5031141..5032091	-	951	WP_182952068.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
H7R89_RS23985	5032084..5032479	-	396	WP_182952069.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
H7R89_RS23990	5032554..5033387	-	834	WP_182952070.1	DUF4942 domain-containing protein	-
H7R89_RS23995	5033501..5033815	-	315	WP_182952493.1	TA system toxin CbtA family protein	Toxin
H7R89_RS24000	5033858..5034181	-	324	WP_182952071.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H7R89_RS24005	5034194..5034664	-	471	WP_047361603.1	DNA repair protein RadC	-
H7R89_RS24010	5034735..5035556	-	822	WP_182952072.1	DUF945 domain-containing protein	-
H7R89_RS24015	5035678..5036130	-	453	WP_182952073.1	hypothetical protein	-
H7R89_RS24020	5036127..5036579	-	453	WP_182952074.1	hypothetical protein	-
H7R89_RS24025	5036617..5037183	-	567	WP_182952075.1	hypothetical protein	-
H7R89_RS24030	5037189..5037890	-	702	WP_182952076.1	WYL domain-containing protein	-
H7R89_RS24035	5038103..5038978	-	876	WP_182952077.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5028327..5062561	34234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (9-103)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11745.33 Da
• Isoelectric Point: 5.0219

>T34020 WP_182952493.1 NZ_AP021983:c5033815-5033501 [Raoultella ornithinolytica]
ISTVPAKVPVSSRPSPVQIWQQLLTYLLDHHYGLTLNDTPFHDDAAIEEHIDAGITLADAVNFLVERYELVRIDCKGFSW
QEQTPFLTVTDILRARRATGLINT

Nucleotide

• Length: 315 bp

>T34020 NZ_AP021983:c5033815-5033501 [Raoultella ornithinolytica]
ATTTCAACTGTACCGGCCAAGGTGCCGGTTTCGTCACGTCCGTCGCCCGTGCAGATCTGGCAGCAACTTTTAACGTATCT
GCTGGATCACCATTACGGCCTGACGCTTAACGACACACCATTCCATGACGACGCGGCCATCGAGGAGCATATCGATGCAG
GAATCACGCTCGCCGATGCTGTTAATTTTCTTGTGGAACGTTATGAACTGGTACGCATCGACTGCAAAGGGTTTAGCTGG
CAGGAACAGACTCCGTTCCTGACCGTGACTGATATTCTCCGAGCCAGGCGAGCTACCGGATTAATAAATACTTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12063.82 Da
• Isoelectric Point: 6.7057

>AT34020 WP_182952071.1 NZ_AP021983:c5034181-5033858 [Raoultella ornithinolytica]
MSITPPHEWGLQSDITPRFGVRLVQEGNRLYYLADRAGLTGGFSPMHLQKLDLAFPLFVEQLEDMLRAGEQNPHQQRQVK
IQLASLTCIADTRGSCGYVYISIYPTP

Nucleotide

• Length: 324 bp

>AT34020 NZ_AP021983:c5034181-5033858 [Raoultella ornithinolytica]
ATGTCAATCACACCACCTCATGAATGGGGTCTACAAAGTGATATCACCCCTCGATTTGGCGTAAGATTAGTTCAGGAAGG
CAACCGGCTGTATTACCTGGCTGACAGGGCTGGATTGACGGGGGGTTTTAGCCCAATGCACCTGCAGAAATTAGATCTGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAGATATGCTGCGTGCTGGCGAACAAAATCCACATCAGCAACGTCAGGTGAAA
ATTCAACTTGCGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGCGGTTACGTTTACATCTCTATTTATCCCACACC
GTAA