Detailed information of TA system TA034019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 5031141..5032479
• Replicon: chromosome
• Accession: NZ_AP021983
• Organism: Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: H7R89_RS23980
• Protein ID: WP_182952068.1
• Coordinates: 5031141..5032091 (-)
• Length: 317 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: H7R89_RS23985
• Protein ID: WP_182952069.1
• Coordinates: 5032084..5032479 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R89_RS23955	5027905..5028237	+	333	WP_071359112.1	endoribonuclease SymE	-
H7R89_RS23960	5028327..5028812	-	486	WP_162661726.1	Hcp family type VI secretion system effector	-
H7R89_RS23965	5028814..5029335	-	522	WP_069476288.1	hypothetical protein	-
H7R89_RS23970	5029316..5029831	-	516	WP_097358169.1	DUF2778 domain-containing protein	-
H7R89_RS23975	5030270..5030967	+	698	Protein_4703	IS1 family transposase	-
H7R89_RS23980	5031141..5032091	-	951	WP_182952068.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
H7R89_RS23985	5032084..5032479	-	396	WP_182952069.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
H7R89_RS23990	5032554..5033387	-	834	WP_182952070.1	DUF4942 domain-containing protein	-
H7R89_RS23995	5033501..5033815	-	315	WP_182952493.1	TA system toxin CbtA family protein	-
H7R89_RS24000	5033858..5034181	-	324	WP_182952071.1	type IV toxin-antitoxin system YeeU family antitoxin	-
H7R89_RS24005	5034194..5034664	-	471	WP_047361603.1	DNA repair protein RadC	-
H7R89_RS24010	5034735..5035556	-	822	WP_182952072.1	DUF945 domain-containing protein	-
H7R89_RS24015	5035678..5036130	-	453	WP_182952073.1	hypothetical protein	-
H7R89_RS24020	5036127..5036579	-	453	WP_182952074.1	hypothetical protein	-
H7R89_RS24025	5036617..5037183	-	567	WP_182952075.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5028327..5062561	34234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-304)

Antitoxin

• Protein of unknown function DUF6088 (7-113)

Sequences

Toxin

Protein

• Length: 317 a.a.
• Molecular weight: 35974.31 Da
• Isoelectric Point: 4.6863

>T34019 WP_182952068.1 NZ_AP021983:c5032091-5031141 [Raoultella ornithinolytica]
MDDLTKLFPDVADALGIESVAIVEKDHYIVELLRLLQPLSFDTHQLVFAGGTALSKAGISLNRMSEDVDIKLVPVAGFSQ
QYSRGQRKSIRKGIIQTIIETIAASDTFRMDEAYSKVTRDEYRYNEIPVRYPQEYVQIPCLRPFIKLELMETDLLEAAEN
RAISSLIMDLTKKGEVVRAFPCATIGSTQAEKLISMMRRTAASMRDVERAVDESLVRHIYDNFCIVREKGADVPVLKRFV
QECIEQDIQRYGAQYPEFCESPVEELKMGLEELASNPVYKERYLQFVTPMVFGESHVSWEEAYACFRITALDVIDA

Nucleotide

• Length: 951 bp

>T34019 NZ_AP021983:c5032091-5031141 [Raoultella ornithinolytica]
ATGGATGATTTAACAAAGCTATTCCCGGACGTTGCTGATGCTCTGGGCATTGAATCAGTTGCAATTGTAGAGAAAGATCA
TTACATCGTTGAGTTATTGCGATTGCTCCAGCCTCTGTCCTTTGATACACATCAGCTGGTTTTTGCCGGTGGAACAGCAT
TGTCTAAAGCGGGTATATCTTTGAACAGGATGTCGGAGGACGTGGACATTAAGCTTGTTCCTGTCGCCGGTTTTTCCCAG
CAATATTCCAGAGGTCAGCGTAAAAGCATCCGTAAGGGTATTATTCAGACCATTATCGAAACGATTGCAGCCTCAGATAC
GTTTCGCATGGATGAAGCGTATTCAAAGGTTACTCGTGATGAATATCGCTATAATGAAATTCCGGTGCGCTACCCGCAGG
AATATGTACAAATTCCCTGTTTAAGGCCATTTATCAAACTGGAACTGATGGAAACGGATTTGCTTGAGGCTGCGGAAAAT
CGTGCTATTAGCTCACTGATAATGGACCTAACAAAGAAGGGCGAAGTAGTGAGGGCTTTTCCCTGCGCAACTATCGGCAG
CACGCAGGCTGAAAAACTGATCTCGATGATGCGCAGAACGGCTGCAAGTATGCGTGATGTGGAGCGTGCAGTGGATGAGT
CACTAGTGCGTCATATCTACGACAACTTTTGTATTGTCAGAGAAAAAGGTGCTGACGTTCCTGTTTTGAAGCGCTTTGTG
CAGGAATGTATTGAGCAGGATATCCAACGTTACGGTGCTCAGTATCCTGAATTCTGCGAGTCGCCTGTTGAAGAACTAAA
AATGGGGTTGGAAGAACTGGCGAGCAACCCTGTTTATAAGGAACGCTATCTGCAGTTTGTTACACCAATGGTGTTCGGTG
AAAGCCACGTTTCCTGGGAAGAGGCCTATGCCTGTTTTCGCATAACGGCATTGGATGTGATTGATGCATAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14341.68 Da
• Isoelectric Point: 10.9938

>AT34019 WP_182952069.1 NZ_AP021983:c5032479-5032084 [Raoultella ornithinolytica]
MTIKARIQSRLKRSKRYVFTRDDFKDLAGYDQIGRALRELMKEGLLLKVGYGVYTKARQNAITGKLMPAAPGGSAAVMIE
ALERLNVDYRLAGATSAYNSGKSTQIPASLEIKTSPRFKRTLSVGGSKLNG

Nucleotide

• Length: 396 bp

>AT34019 NZ_AP021983:c5032479-5032084 [Raoultella ornithinolytica]
ATGACCATAAAAGCTCGTATTCAGTCACGGCTGAAGCGCTCAAAGCGTTATGTTTTCACTCGTGACGATTTCAAAGATCT
TGCTGGCTACGATCAAATAGGCAGAGCACTGCGTGAGCTGATGAAGGAAGGGCTGCTACTGAAAGTAGGCTATGGCGTCT
ACACCAAAGCGCGGCAAAATGCGATTACCGGTAAGCTGATGCCTGCTGCTCCCGGAGGTTCTGCTGCGGTAATGATTGAG
GCGTTGGAACGTCTGAACGTGGATTACCGTCTGGCAGGAGCAACAAGTGCCTACAACAGTGGTAAATCTACCCAGATCCC
CGCGTCACTTGAAATCAAAACCTCCCCCCGTTTCAAGAGAACATTGTCAGTAGGGGGCAGCAAACTAAATGGATGA