Detailed information of TA system TA034018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4942779..4943358
• Replicon: chromosome
• Accession: NZ_AP021983
• Organism: Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: H7R89_RS23580
• Protein ID: WP_118860181.1
• Coordinates: 4943071..4943358 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: H7R89_RS23575
• Protein ID: WP_182952061.1
• Coordinates: 4942779..4943084 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R89_RS23550	4937985..4939049	-	1065	WP_004857527.1	DUF2955 domain-containing protein	-
H7R89_RS23555	4939039..4940106	-	1068	WP_004857525.1	HlyD family secretion protein	-
H7R89_RS23560	4940113..4940577	-	465	WP_004857523.1	MarR family transcriptional regulator	-
H7R89_RS23565	4940869..4941033	-	165	WP_004857521.1	DUF1127 domain-containing protein	-
H7R89_RS23570	4941211..4942623	+	1413	WP_015585221.1	PLP-dependent aminotransferase family protein	-
H7R89_RS23575	4942779..4943084	-	306	WP_182952061.1	BrnA antitoxin family protein	Antitoxin
H7R89_RS23580	4943071..4943358	-	288	WP_118860181.1	BrnT family toxin	Toxin
H7R89_RS23585	4943476..4943895	-	420	WP_127673703.1	FosA family fosfomycin resistance glutathione transferase	-
H7R89_RS23590	4943889..4944803	-	915	WP_182952062.1	LysR family transcriptional regulator	-
H7R89_RS23595	4944891..4945673	+	783	Protein_4631	NAD(P)H-dependent oxidoreductase	-
H7R89_RS23600	4945820..4946404	+	585	WP_104895470.1	TetR/AcrR family transcriptional regulator	-
H7R89_RS23605	4946548..4947360	+	813	WP_104896593.1	winged helix-turn-helix domain-containing protein	-
H7R89_RS23610	4947357..4947875	+	519	WP_015585229.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (34-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11157.65 Da
• Isoelectric Point: 7.5236

>T34018 WP_118860181.1 NZ_AP021983:c4943358-4943071 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHERYENGEYRWQTIGLVHGIIVILVVHSVRFESGAEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T34018 NZ_AP021983:c4943358-4943071 [Raoultella ornithinolytica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGACATGAACGCTATGAAAATGGTGAGTATCGATGGCAAACCATCGGCC
TCGTTCATGGAATTATCGTTATTCTTGTGGTGCATTCGGTCCGCTTTGAAAGCGGGGCTGAGGTGATTCGTATCATCAGC
GCCCGGAAGGCGGACAAAAAAGAGAGGAGTCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11709.23 Da
• Isoelectric Point: 9.4631

>AT34018 WP_182952061.1 NZ_AP021983:c4943084-4942779 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALARKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKKS

Nucleotide

• Length: 306 bp

>AT34018 NZ_AP021983:c4943084-4942779 [Raoultella ornithinolytica]
ATGAGCATGGTTAAACATAAACGCGATAACCCGACAGCGCTTACCGCTCAGCGTGAAGACGAACTCAAAGCGCTGGCGAG
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGTGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAGACCCGGCTGAACGCGATTCTGCGGGAAGCCATGCTGCGCGAGCAGAATAAGAAATCGTAA