34017

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4349858..4350477 Replicon chromosome
Accession NZ_AP021983
Organism Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag H7R89_RS20745 Protein ID WP_004858783.1
Coordinates 4350259..4350477 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag H7R89_RS20740 Protein ID WP_004858785.1
Coordinates 4349858..4350232 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R89_RS20730 4344968..4346161 + 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7R89_RS20735 4346184..4349330 + 3147 WP_004858787.1 multidrug efflux RND transporter permease subunit -
H7R89_RS20740 4349858..4350232 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
H7R89_RS20745 4350259..4350477 + 219 WP_004858783.1 hemolysin expression modulator Hha Toxin
H7R89_RS20750 4350629..4351195 + 567 WP_004858781.1 maltose O-acetyltransferase -
H7R89_RS20755 4351295..4351765 + 471 WP_004858780.1 YlaC family protein -
H7R89_RS20760 4351740..4353191 - 1452 WP_104896844.1 PLP-dependent aminotransferase family protein -
H7R89_RS20765 4353295..4354005 + 711 WP_004858778.1 GNAT family N-acetyltransferase -
H7R89_RS20770 4354002..4354142 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
H7R89_RS20775 4354145..4354405 - 261 WP_004858777.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T34017 WP_004858783.1 NZ_AP021983:4350259-4350477 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T34017 NZ_AP021983:4350259-4350477 [Raoultella ornithinolytica]
ATGTCTGGTGAACCATTAACCAAAACAGACTATTTGATGCGCTTACGTCGCTGTCAGACAATTGATACGCTGGAACGCGT
AATTGAAAAAAACAAATATGAACTTTCAGATAACGAACTGGCTGTATTTTACTCAGCGGCCGACCACCGTCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAAATCCCGACATCGGTGTGGAAGTTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT34017 WP_004858785.1 NZ_AP021983:4349858-4350232 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT34017 NZ_AP021983:4349858-4350232 [Raoultella ornithinolytica]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCGCAGCTTCGATTTCTTTGCGAAACCCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGTAACCACGGGTGGGTGAATGATCCAACGTCGGCTATCAATCTTCAACTTAATGAATTGATCGAAC
ACATAGCCACGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAACTGATCGCTCAGCTGGACGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGCATTAACACGGCCGATCTACAGAAATGGCGCAAATCGGGAAATCGGTT
ATTCCGATGTTTTGTCAACGCCAGTCGGGAAAATCCGGCAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References