34004

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 70377..70924 Replicon chromosome
Accession NZ_AP021983
Organism Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)


Gene name ccdB Uniprot ID A0A808XUV0
Locus tag H7R89_RS00330 Protein ID WP_048025274.1
Coordinates 70377..70685 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A808XYT1
Locus tag H7R89_RS00335 Protein ID WP_004869059.1
Coordinates 70688..70924 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R89_RS00300 65556..65858 - 303 WP_015585486.1 PTS lactose/cellobiose transporter subunit IIA -
H7R89_RS00305 65993..67318 - 1326 WP_182952115.1 PTS transporter subunit EIIC -
H7R89_RS00310 67330..67644 - 315 WP_004869063.1 PTS sugar transporter subunit IIB -
H7R89_RS00315 67939..68880 + 942 WP_015585487.1 LacI family transcriptional regulator -
H7R89_RS00320 68975..69250 - 276 WP_160873538.1 hypothetical protein -
H7R89_RS00325 69359..70261 - 903 WP_015585496.1 EamA family transporter -
H7R89_RS00330 70377..70685 - 309 WP_048025274.1 CcdB family protein Toxin
H7R89_RS00335 70688..70924 - 237 WP_004869059.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H7R89_RS00340 71030..72463 - 1434 WP_069476354.1 PTS N-acetylmuramic acid transporter subunit IIBC -
H7R89_RS00345 72488..73390 - 903 WP_088402359.1 N-acetylmuramic acid 6-phosphate etherase -
H7R89_RS00350 73555..74718 - 1164 WP_004869055.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11778.62 Da        Isoelectric Point: 6.7156

>T34004 WP_048025274.1 NZ_AP021983:c70685-70377 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Download         Length: 309 bp

>T34004 NZ_AP021983:c70685-70377 [Raoultella ornithinolytica]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGGTCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCGG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8898.08 Da        Isoelectric Point: 5.6579

>AT34004 WP_004869059.1 NZ_AP021983:c70924-70688 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT34004 NZ_AP021983:c70924-70688 [Raoultella ornithinolytica]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808XUV0

Antitoxin

Source ID Structure
AlphaFold DB A0A808XYT1

References