Detailed information of TA system TA034002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 107371..108014
• Replicon: plasmid pWP3-W18-ESBL-02_2
• Accession: NZ_AP021974
• Organism: Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: H7R56_RS26800
• Protein ID: WP_182928842.1
• Coordinates: 107598..108014 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: H7R56_RS26795
• Protein ID: WP_001261282.1
• Coordinates: 107371..107601 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R56_RS26755	102592..102894	+	303	WP_071571079.1	hypothetical protein	-
H7R56_RS26760	103334..104128	-	795	WP_004197635.1	site-specific integrase	-
H7R56_RS26765	104326..105342	-	1017	WP_017899884.1	hypothetical protein	-
H7R56_RS26770	105353..105667	-	315	WP_053389906.1	hypothetical protein	-
H7R56_RS26775	105694..106089	-	396	WP_017899885.1	hypothetical protein	-
H7R56_RS26780	106258..106563	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
H7R56_RS26785	106565..106783	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
H7R56_RS26790	106953..107414	-	462	Protein_127	hypothetical protein	-
H7R56_RS26795	107371..107601	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H7R56_RS26800	107598..108014	+	417	WP_182928842.1	type II toxin-antitoxin system VapC family toxin	Toxin
H7R56_RS26805	108088..108324	+	237	WP_001348075.1	AAA family ATPase	-
H7R56_RS26810	108371..109075	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H7R56_RS26815	109130..110713	+	1584	Protein_132	Tn3 family transposase	-
H7R56_RS26820	110792..111796	+	1005	WP_022542389.1	IS110-like element IS4321 family transposase	-
H7R56_RS26825	112171..112437	+	267	WP_001567383.1	DUF1471 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..146141	146141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15118.57 Da
• Isoelectric Point: 7.1084

>T34002 WP_182928842.1 NZ_AP021974:107598-108014 [Klebsiella sp. WP3-W18-ESBL-02]
VNKIYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T34002 NZ_AP021974:107598-108014 [Klebsiella sp. WP3-W18-ESBL-02]
GTGAACAAGATCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT34002 WP_001261282.1 NZ_AP021974:107371-107601 [Klebsiella sp. WP3-W18-ESBL-02]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT34002 NZ_AP021974:107371-107601 [Klebsiella sp. WP3-W18-ESBL-02]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3