Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 106258..106783 Replicon plasmid pWP3-W18-ESBL-02_2
Accession NZ_AP021974
Organism Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag H7R56_RS26780 Protein ID WP_013023785.1
Coordinates 106258..106563 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag H7R56_RS26785 Protein ID WP_001568025.1
Coordinates 106565..106783 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R56_RS26750 101969..102595 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
H7R56_RS26755 102592..102894 + 303 WP_071571079.1 hypothetical protein -
H7R56_RS26760 103334..104128 - 795 WP_004197635.1 site-specific integrase -
H7R56_RS26765 104326..105342 - 1017 WP_017899884.1 hypothetical protein -
H7R56_RS26770 105353..105667 - 315 WP_053389906.1 hypothetical protein -
H7R56_RS26775 105694..106089 - 396 WP_017899885.1 hypothetical protein -
H7R56_RS26780 106258..106563 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R56_RS26785 106565..106783 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R56_RS26790 106953..107414 - 462 Protein_127 hypothetical protein -
H7R56_RS26795 107371..107601 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R56_RS26800 107598..108014 + 417 WP_182928842.1 type II toxin-antitoxin system VapC family toxin -
H7R56_RS26805 108088..108324 + 237 WP_001348075.1 AAA family ATPase -
H7R56_RS26810 108371..109075 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
H7R56_RS26815 109130..110713 + 1584 Protein_132 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..146141 146141


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T34001 WP_013023785.1 NZ_AP021974:c106563-106258 [Klebsiella sp. WP3-W18-ESBL-02]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34001 NZ_AP021974:c106563-106258 [Klebsiella sp. WP3-W18-ESBL-02]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT34001 WP_001568025.1 NZ_AP021974:c106783-106565 [Klebsiella sp. WP3-W18-ESBL-02]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34001 NZ_AP021974:c106783-106565 [Klebsiella sp. WP3-W18-ESBL-02]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References