33999

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 25948..26474 Replicon plasmid pWP3-W18-ESBL-02_2
Accession NZ_AP021974
Organism Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H7R56_RS26330 Protein ID WP_000323025.1
Coordinates 25948..26235 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H7R56_RS26335 Protein ID WP_000534858.1
Coordinates 26235..26474 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R56_RS26290 21639..21845 - 207 WP_182928863.1 hypothetical protein -
H7R56_RS26295 21845..22165 - 321 WP_182928864.1 hypothetical protein -
H7R56_RS26300 22227..22661 - 435 WP_182928882.1 hypothetical protein -
H7R56_RS26305 23462..23788 - 327 WP_182928865.1 hypothetical protein -
H7R56_RS26310 23860..24006 - 147 WP_182928866.1 succinate dehydrogenase flavoprotein subunit -
H7R56_RS26315 24094..24561 - 468 WP_182928867.1 hypothetical protein -
H7R56_RS26320 24606..24917 - 312 WP_182928868.1 hypothetical protein -
H7R56_RS26325 25718..25876 - 159 WP_182928869.1 type I toxin-antitoxin system Hok family toxin -
H7R56_RS26330 25948..26235 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H7R56_RS26335 26235..26474 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H7R56_RS26340 26737..27660 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
H7R56_RS26345 27860..28264 - 405 Protein_38 cytochrome b/b6 domain-containing protein -
H7R56_RS26355 30122..30927 + 806 Protein_40 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..146141 146141
- flank IS/Tn - - 30427..30927 500


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T33999 WP_000323025.1 NZ_AP021974:c26235-25948 [Klebsiella sp. WP3-W18-ESBL-02]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T33999 NZ_AP021974:c26235-25948 [Klebsiella sp. WP3-W18-ESBL-02]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT33999 WP_000534858.1 NZ_AP021974:c26474-26235 [Klebsiella sp. WP3-W18-ESBL-02]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT33999 NZ_AP021974:c26474-26235 [Klebsiella sp. WP3-W18-ESBL-02]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References