Detailed information of TA system TA033998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 178205..178739
• Replicon: plasmid pWP3-W18-ESBL-02_1
• Accession: NZ_AP021973
• Organism: Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H7R56_RS25875
• Protein ID: WP_123282529.1
• Coordinates: 178205..178489 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: H7R56_RS25880
• Protein ID: WP_123282528.1
• Coordinates: 178476..178739 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R56_RS25860	173513..173740	+	228	WP_007372341.1	hypothetical protein	-
H7R56_RS25865	173921..174844	+	924	WP_007372342.1	hypothetical protein	-
H7R56_RS25870	175038..178118	-	3081	WP_182928827.1	Tn3 family transposase	-
H7R56_RS25875	178205..178489	-	285	WP_123282529.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H7R56_RS25880	178476..178739	-	264	WP_123282528.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H7R56_RS25885	178917..179165	+	249	WP_012585400.1	type II toxin-antitoxin system YafQ family toxin	-
H7R56_RS25890	179292..179867	+	576	WP_001173919.1	recombinase family protein	-
H7R56_RS25895	179989..180591	+	603	WP_010465829.1	recombinase family protein	-
H7R56_RS25900	180575..183604	+	3030	WP_182928828.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..230471	230471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-70)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10843.47 Da
• Isoelectric Point: 8.0737

>T33998 WP_123282529.1 NZ_AP021973:c178489-178205 [Klebsiella sp. WP3-W18-ESBL-02]
MRTIEYTSQFKRDFKRESKGQYKAVLAGAEFSLIVQVLANDLPLPEKLRDHPLSGDWKDHRDCHVKPDLVLIYRKPDDTV
LQLVRLGSHSELGL

Nucleotide

• Length: 285 bp

>T33998 NZ_AP021973:c178489-178205 [Klebsiella sp. WP3-W18-ESBL-02]
ATGCGGACGATTGAATACACAAGCCAGTTCAAGCGAGATTTTAAACGCGAAAGCAAAGGGCAATACAAAGCGGTTCTTGC
CGGTGCCGAGTTTTCGTTGATAGTTCAAGTTTTGGCAAATGATTTGCCATTGCCTGAAAAACTACGCGATCACCCGCTCT
CAGGTGACTGGAAAGATCATCGGGACTGTCATGTTAAGCCCGATCTGGTTTTAATTTACCGAAAACCAGATGACACCGTA
TTGCAGCTTGTTCGTTTAGGGTCACATAGCGAATTAGGGTTATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9487.95 Da
• Isoelectric Point: 4.6286

>AT33998 WP_123282528.1 NZ_AP021973:c178739-178476 [Klebsiella sp. WP3-W18-ESBL-02]
MSANAVVRARIDEHIKEEATIVLASMGLTVSDAFRIMLTRVAREKALPFEPLVPNAETIEAMKEARKGGLKSFATVDDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT33998 NZ_AP021973:c178739-178476 [Klebsiella sp. WP3-W18-ESBL-02]
ATGTCTGCAAATGCCGTTGTACGTGCCCGTATTGATGAACACATCAAAGAAGAAGCTACTATCGTTCTGGCATCGATGGG
GCTGACTGTATCTGATGCTTTCCGGATCATGCTGACTCGTGTTGCCCGTGAAAAAGCATTGCCATTCGAGCCATTGGTAC
CCAATGCTGAAACTATTGAAGCTATGAAAGAAGCTCGTAAAGGTGGGTTGAAATCTTTCGCAACAGTTGATGATTTAATG
GCTGATCTCAATGCGGACGATTGA