Detailed information of TA system TA033996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 125119..125669
• Replicon: plasmid pWP3-W18-ESBL-02_1
• Accession: NZ_AP021973
• Organism: Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: H7R56_RS25550
• Protein ID: WP_007372286.1
• Coordinates: 125361..125669 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: H7R56_RS25545
• Protein ID: WP_007372285.1
• Coordinates: 125119..125358 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R56_RS25500	120126..120521	+	396	WP_182928823.1	hypothetical protein	-
H7R56_RS25505	120703..120894	+	192	WP_016241560.1	hypothetical protein	-
H7R56_RS25510	121025..121720	+	696	WP_007372278.1	hypothetical protein	-
H7R56_RS25515	121743..121940	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
H7R56_RS25520	121993..122451	+	459	WP_182928824.1	hypothetical protein	-
H7R56_RS25525	122506..122739	+	234	WP_008786598.1	hypothetical protein	-
H7R56_RS25530	122755..123387	+	633	WP_007372282.1	hypothetical protein	-
H7R56_RS25535	123848..124519	+	672	WP_007372283.1	hypothetical protein	-
H7R56_RS25540	124516..124980	+	465	WP_008786596.1	hypothetical protein	-
H7R56_RS25545	125119..125358	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H7R56_RS25550	125361..125669	+	309	WP_007372286.1	CcdB family protein	Toxin
H7R56_RS25555	125690..125848	+	159	WP_016241558.1	hypothetical protein	-
H7R56_RS25560	126003..126557	+	555	WP_007372288.1	hypothetical protein	-
H7R56_RS25565	126627..127244	+	618	WP_007372289.1	hypothetical protein	-
H7R56_RS25570	127327..127797	+	471	WP_007372290.1	hypothetical protein	-
H7R56_RS25575	128144..128386	+	243	WP_007372291.1	hypothetical protein	-
H7R56_RS25580	128397..128795	+	399	WP_007372292.1	hypothetical protein	-
H7R56_RS25585	128879..129205	+	327	WP_007372293.1	hypothetical protein	-
H7R56_RS25590	129568..129906	-	339	WP_007372294.1	hypothetical protein	-
H7R56_RS25595	130144..130587	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..230471	230471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T33996 WP_007372286.1 NZ_AP021973:125361-125669 [Klebsiella sp. WP3-W18-ESBL-02]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T33996 NZ_AP021973:125361-125669 [Klebsiella sp. WP3-W18-ESBL-02]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT33996 WP_007372285.1 NZ_AP021973:125119-125358 [Klebsiella sp. WP3-W18-ESBL-02]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT33996 NZ_AP021973:125119-125358 [Klebsiella sp. WP3-W18-ESBL-02]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3