Detailed information of TA system TA033995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5079676..5080292
• Replicon: chromosome
• Accession: NZ_AP021972
• Organism: Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H7R56_RS24390
• Protein ID: WP_106929282.1
• Coordinates: 5079676..5080047 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H7R56_RS24395
• Protein ID: WP_181358031.1
• Coordinates: 5080044..5080292 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R56_RS24375	5077180..5078082	+	903	WP_106929286.1	formate dehydrogenase subunit beta	-
H7R56_RS24380	5078079..5078714	+	636	WP_006179126.1	formate dehydrogenase cytochrome b556 subunit	-
H7R56_RS24385	5078711..5079640	+	930	WP_106929284.1	formate dehydrogenase accessory protein FdhE	-
H7R56_RS24390	5079676..5080047	-	372	WP_106929282.1	type II toxin-antitoxin system VapC family toxin	Toxin
H7R56_RS24395	5080044..5080292	-	249	WP_181358031.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R56_RS24400	5080498..5081409	+	912	WP_106929280.1	alpha/beta hydrolase	-
H7R56_RS24405	5081433..5082374	-	942	WP_181358030.1	fatty acid biosynthesis protein FabY	-
H7R56_RS24410	5082419..5082856	-	438	WP_106929276.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7R56_RS24415	5082853..5083719	-	867	WP_106929274.1	virulence factor BrkB family protein	-
H7R56_RS24420	5083713..5084312	-	600	WP_106929272.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5070618..5080298	9680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14118.53 Da
• Isoelectric Point: 9.5896

>T33995 WP_106929282.1 NZ_AP021972:c5080047-5079676 [Klebsiella sp. WP3-W18-ESBL-02]
MKRPVLFDTNILIDLFSGRMEAKRAMDSYPPQHAISLITWMEVMIGAKRFHQEQRTRIALSAFEIIGVTEEIAEQSVNLR
QEYGMKLPDAIILATAHVHRRELVTRNTKDFARLPGVILPYAL

Nucleotide

• Length: 372 bp

>T33995 NZ_AP021972:c5080047-5079676 [Klebsiella sp. WP3-W18-ESBL-02]
ATGAAAAGACCCGTTCTTTTTGATACCAATATTTTAATCGATCTCTTCAGCGGGCGGATGGAGGCGAAGCGGGCGATGGA
TTCCTACCCTCCGCAACATGCGATAAGCCTGATCACCTGGATGGAGGTCATGATAGGGGCAAAGCGCTTTCATCAGGAGC
AGCGAACGCGAATAGCCTTGAGCGCATTCGAAATTATTGGTGTGACAGAAGAAATTGCTGAGCAGAGTGTGAATCTACGG
CAGGAGTACGGCATGAAGCTGCCCGATGCGATTATTCTGGCCACTGCGCATGTTCATCGCCGGGAACTGGTGACGCGTAA
TACAAAGGATTTTGCGAGGCTTCCGGGCGTGATTTTACCCTACGCGCTGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9746.13 Da
• Isoelectric Point: 4.4777

>AT33995 WP_181358031.1 NZ_AP021972:c5080292-5080044 [Klebsiella sp. WP3-W18-ESBL-02]
MMVERDMGRILLDLSDEVIKRLDILKQQRNLPRAELLREAVEQYLEKQSQAELTISGALGIWQGCEEDGVEYERKLREEW
QR

Nucleotide

• Length: 249 bp

>AT33995 NZ_AP021972:c5080292-5080044 [Klebsiella sp. WP3-W18-ESBL-02]
ATGATGGTGGAAAGGGACATGGGCAGAATACTTCTCGATTTGTCGGACGAGGTGATTAAGCGCCTCGACATACTTAAACA
GCAGCGAAATCTGCCTCGTGCGGAACTGCTGCGTGAAGCCGTTGAGCAATATCTTGAAAAGCAGAGTCAGGCTGAGCTCA
CTATTTCTGGCGCGCTTGGCATCTGGCAAGGGTGTGAAGAAGATGGCGTTGAGTATGAAAGAAAACTTCGGGAAGAGTGG
CAGCGATGA