Detailed information of TA system TA033992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4433634..4434210
• Replicon: chromosome
• Accession: NZ_AP021972
• Organism: Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: H7R56_RS21375
• Protein ID: WP_106930463.1
• Coordinates: 4433923..4434210 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: H7R56_RS21370
• Protein ID: WP_106930461.1
• Coordinates: 4433634..4433936 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R56_RS21350	4430361..4431104	+	744	WP_106930457.1	AraC family transcriptional regulator	-
H7R56_RS21355	4431152..4431793	+	642	WP_106930459.1	LysE family translocator	-
H7R56_RS21360	4431834..4431998	-	165	WP_072011371.1	DUF1127 domain-containing protein	-
H7R56_RS21365	4432177..4433589	+	1413	WP_035893387.1	PLP-dependent aminotransferase family protein	-
H7R56_RS21370	4433634..4433936	-	303	WP_106930461.1	BrnA antitoxin family protein	Antitoxin
H7R56_RS21375	4433923..4434210	-	288	WP_106930463.1	BrnT family toxin	Toxin
H7R56_RS21380	4434439..4435101	-	663	WP_106930465.1	hypothetical protein	-
H7R56_RS21385	4435263..4435600	+	338	Protein_4192	endoribonuclease SymE	-
H7R56_RS21390	4435659..4436603	-	945	WP_106930467.1	TauD/TfdA family dioxygenase	-
H7R56_RS21395	4436808..4437050	+	243	WP_106930469.1	DUF2164 family protein	-
H7R56_RS21400	4437047..4437517	+	471	WP_106930471.1	GNAT family N-acetyltransferase	-
H7R56_RS21405	4437603..4438679	-	1077	WP_106930473.1	glycoside hydrolase family 88 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11178.54 Da
• Isoelectric Point: 6.9900

>T33992 WP_106930463.1 NZ_AP021972:c4434210-4433923 [Klebsiella sp. WP3-W18-ESBL-02]
MPMEFEWDANKAQSNLRKHGVRFEDAVLVFDDPQHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVQFESGHEVIRIIS
ARKADRKERSRYEQG

Nucleotide

• Length: 288 bp

>T33992 NZ_AP021972:c4434210-4433923 [Klebsiella sp. WP3-W18-ESBL-02]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCACAAAGTAACCTGAGGAAACACGGTGTCCGCTTTGAGGATGCGGT
GCTGGTATTTGACGATCCCCAACATCTGTCGCGGCAGGATCGGTATGAGAACGGCGAGTATCGCTGGCAGACCATCGGTC
TTGTTCATGGCATTGTGGTTATTCTGGTTGCCCACAGCGTTCAGTTCGAAAGTGGTCATGAAGTGATTCGCATCATCAGC
GCCAGAAAGGCAGACCGTAAAGAGAGGAGTCGTTATGAACAGGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11570.06 Da
• Isoelectric Point: 9.9257

>AT33992 WP_106930461.1 NZ_AP021972:c4433936-4433634 [Klebsiella sp. WP3-W18-ESBL-02]
MNRVKHKRGSVSTLSPQHEAELKALANKSDDEIDYSDIPATEDEQWSEANRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT33992 NZ_AP021972:c4433936-4433634 [Klebsiella sp. WP3-W18-ESBL-02]
ATGAACAGGGTTAAGCATAAACGCGGCAGCGTATCCACGCTGAGTCCTCAGCATGAGGCTGAACTGAAAGCGCTGGCGAA
TAAATCGGATGATGAAATTGATTACAGCGACATTCCTGCGACAGAAGATGAGCAGTGGTCAGAGGCTAATCGCGGTAAGT
TTTTCCGTCCGTTGAAAACGCAGGCGTCTGTGCGCATTGATGCTGACGTGATGGAATGGTTGAAACGCCCTGGAAAGGGT
TACCAGACGCGGCTCAACGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAA