Detailed information of TA system TA033990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3734069..3734687
• Replicon: chromosome
• Accession: NZ_AP021972
• Organism: Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1B7JI95
• Locus tag: H7R56_RS17820
• Protein ID: WP_064547995.1
• Coordinates: 3734469..3734687 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: H7R56_RS17815
• Protein ID: WP_106927922.1
• Coordinates: 3734069..3734443 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R56_RS17805	3729168..3730364	+	1197	WP_106930570.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
H7R56_RS17810	3730387..3733533	+	3147	WP_106930572.1	multidrug efflux RND transporter permease subunit AcrB	-
H7R56_RS17815	3734069..3734443	+	375	WP_106927922.1	Hha toxicity modulator TomB	Antitoxin
H7R56_RS17820	3734469..3734687	+	219	WP_064547995.1	hemolysin expression modulator Hha	Toxin
H7R56_RS17825	3734820..3735377	+	558	WP_106927924.1	maltose O-acetyltransferase	-
H7R56_RS17830	3735480..3735950	+	471	WP_106927926.1	hypothetical protein	-
H7R56_RS17835	3735919..3737376	-	1458	WP_106927928.1	PLP-dependent aminotransferase family protein	-
H7R56_RS17840	3737480..3738178	+	699	WP_106927930.1	GNAT family N-acetyltransferase	-
H7R56_RS17845	3738175..3738315	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
H7R56_RS17850	3738315..3738578	-	264	WP_106927932.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8629.97 Da
• Isoelectric Point: 8.8580

>T33990 WP_064547995.1 NZ_AP021972:3734469-3734687 [Klebsiella sp. WP3-W18-ESBL-02]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSTVWKYIR

Nucleotide

• Length: 219 bp

>T33990 NZ_AP021972:3734469-3734687 [Klebsiella sp. WP3-W18-ESBL-02]
ATGTCTGATAAACCACTAACTAAAACTGATTATTTGATGCGCCTGCGACGCTGCCAGTCTATTGACACGCTCGAACGCGT
AATTGAAAAAAATAAATATGAGCTGTCCGACAATGAACTGGCGGTATTTTACTCAGCGGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAACTCTACGATAAGATCCCTTCAACCGTGTGGAAGTACATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14383.21 Da
• Isoelectric Point: 4.8886

>AT33990 WP_106927922.1 NZ_AP021972:3734069-3734443 [Klebsiella sp. WP3-W18-ESBL-02]
MDEYSPKRHDIAQLKFLCETLYHDCLATLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYDEDNKLIAQIDEYL
DDTFMLFSNYGINSVDLQKWRKSGNKLFRCFVNASRANPVSLSC

Nucleotide

• Length: 375 bp

>AT33990 NZ_AP021972:3734069-3734443 [Klebsiella sp. WP3-W18-ESBL-02]
ATGGACGAATACTCACCAAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGCGAAACCCTGTATCATGATTGTCTTGC
CACGCTCGAAGAGAGTAACCATGGCTGGGTGAACGATCCCACCTCGGCGGTTAACCTGCAGCTTAATGAGCTGATAGAAC
ATATAGCAACGTTCGCACTTAATTATAAAATTAAGTACGACGAAGATAATAAACTGATTGCACAGATTGACGAATATCTG
GATGATACTTTTATGTTATTCAGTAACTACGGCATTAATAGCGTCGACCTGCAAAAATGGCGGAAGTCAGGGAACAAACT
CTTCCGTTGTTTTGTCAATGCGAGCCGCGCCAACCCCGTCAGTCTTTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1B7JI95