Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1109511..1110037 Replicon chromosome
Accession NZ_AP021972
Organism Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H7R56_RS05365 Protein ID WP_000323025.1
Coordinates 1109511..1109798 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H7R56_RS05370 Protein ID WP_000534858.1
Coordinates 1109798..1110037 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R56_RS05340 1105265..1106257 + 993 WP_106926080.1 RNA polymerase sigma factor RpoS -
H7R56_RS05345 1106319..1106723 - 405 WP_106926082.1 MarR family transcriptional regulator -
H7R56_RS05350 1106865..1107458 + 594 WP_106926084.1 UbiX family flavin prenyltransferase -
H7R56_RS05355 1107458..1108885 + 1428 WP_106926086.1 UbiD family decarboxylase -
H7R56_RS05360 1108896..1109132 + 237 WP_106926088.1 hypothetical protein -
H7R56_RS05365 1109511..1109798 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H7R56_RS05370 1109798..1110037 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H7R56_RS05375 1110300..1111223 - 924 Protein_1055 cation transporter -
H7R56_RS05380 1111423..1111995 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
H7R56_RS05385 1112638..1113978 - 1341 WP_000589001.1 ISNCY family transposase -
H7R56_RS05390 1114056..1114208 + 153 WP_106926090.1 type I toxin-antitoxin system Hok family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T33978 WP_000323025.1 NZ_AP021972:c1109798-1109511 [Klebsiella sp. WP3-W18-ESBL-02]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T33978 NZ_AP021972:c1109798-1109511 [Klebsiella sp. WP3-W18-ESBL-02]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT33978 WP_000534858.1 NZ_AP021972:c1110037-1109798 [Klebsiella sp. WP3-W18-ESBL-02]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT33978 NZ_AP021972:c1110037-1109798 [Klebsiella sp. WP3-W18-ESBL-02]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References