Detailed information of TA system TA033974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 385760..386346
• Replicon: chromosome
• Accession: NZ_AP021972
• Organism: Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: H7R56_RS01870
• Protein ID: WP_106925089.1
• Coordinates: 385978..386346 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: H7R56_RS01865
• Protein ID: WP_181357971.1
• Coordinates: 385760..385981 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R56_RS01840	380815..381924	+	1110	WP_106925084.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
H7R56_RS01845	381979..382905	+	927	WP_106925085.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
H7R56_RS01850	382902..384182	+	1281	WP_106925086.1	branched chain amino acid ABC transporter permease LivM	-
H7R56_RS01855	384179..384946	+	768	WP_106925087.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
H7R56_RS01860	384948..385661	+	714	WP_106925088.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
H7R56_RS01865	385760..385981	+	222	WP_181357971.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H7R56_RS01870	385978..386346	+	369	WP_106925089.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H7R56_RS01875	386579..387895	+	1317	WP_106925090.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
H7R56_RS01880	387996..388883	+	888	WP_106925091.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
H7R56_RS01885	388880..389725	+	846	WP_106925092.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
H7R56_RS01890	389728..390798	+	1071	WP_106925093.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	374082..391535	17453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13526.80 Da
• Isoelectric Point: 6.7249

>T33974 WP_106925089.1 NZ_AP021972:385978-386346 [Klebsiella sp. WP3-W18-ESBL-02]
MTLQIISAEEIIQFHDRLLRVTPGVEGMPDPGRAEALMYRVLNQIEYQGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLPANPDFVAMTVEAAAGQLTLEQIAARLRG

Nucleotide

• Length: 369 bp

>T33974 NZ_AP021972:385978-386346 [Klebsiella sp. WP3-W18-ESBL-02]
ATGACCCTTCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGTTGCTCCGCGTAACGCCTGGCGTAGAGGG
AATGCCCGATCCTGGCCGCGCAGAAGCGCTGATGTACCGGGTGCTCAATCAAATCGAATATCAAGGCGTGACAGATGTCT
GGCTGCTTGCGGCAATGCATCTGCTGGCGATTTCGCGTGGGCATATTTTTAATGACGGAAATAAACGGACGGCATTATTT
ATTACCTTACTGTTTTTAAAGCGTAACGGCATTTCATTACCGGCTAATCCTGATTTTGTTGCGATGACGGTTGAGGCTGC
CGCTGGGCAACTCACGCTGGAGCAGATAGCAGCCCGGCTCCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8146.18 Da
• Isoelectric Point: 5.0775

>AT33974 WP_181357971.1 NZ_AP021972:385760-385981 [Klebsiella sp. WP3-W18-ESBL-02]
MRTINYSEARQNLAEVLESAVSGGPVTITRRGHKPAVIISAEEFERYQAARMDDEFAAIMAIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT33974 NZ_AP021972:385760-385981 [Klebsiella sp. WP3-W18-ESBL-02]
ATGCGTACGATTAACTACAGTGAAGCGCGGCAGAATCTGGCGGAGGTACTGGAAAGTGCCGTCAGCGGGGGGCCTGTCAC
CATTACGCGCCGAGGGCATAAGCCTGCGGTGATTATCAGCGCGGAAGAATTTGAGCGTTATCAGGCGGCCAGAATGGATG
ATGAATTCGCGGCAATCATGGCGATACATGGTAATGAAATCAGGGAGCTGGCAGATAAATGA