Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 101..623 Replicon plasmid pWP3-W18-CRE-03_2
Accession NZ_AP021965
Organism Escherichia coli strain WP3-W18-CRE-03

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag H7R02_RS25000 Protein ID WP_000220561.1
Coordinates 342..623 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag H7R02_RS24995 Protein ID WP_000121743.1
Coordinates 101..352 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R02_RS24995 101..352 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
H7R02_RS25000 342..623 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R02_RS25005 973..1488 + 516 WP_096836718.1 DnaJ domain-containing protein -
H7R02_RS25010 1562..2110 + 549 WP_096836696.1 hypothetical protein -
H7R02_RS25015 2176..2388 + 213 WP_001446884.1 hypothetical protein -
H7R02_RS25020 2378..2620 + 243 WP_000008708.1 hypothetical protein -
H7R02_RS25025 2714..3043 + 330 WP_096836693.1 hypothetical protein -
H7R02_RS25030 3086..3265 + 180 WP_000439078.1 hypothetical protein -
H7R02_RS25035 3335..3484 + 150 WP_000003880.1 hypothetical protein -
H7R02_RS25040 3497..3769 + 273 WP_000160399.1 hypothetical protein -
H7R02_RS25045 4085..4630 + 546 WP_135167680.1 DNA distortion polypeptide 1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..47584 47584


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T33973 WP_000220561.1 NZ_AP021965:342-623 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T33973 NZ_AP021965:342-623 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT33973 WP_000121743.1 NZ_AP021965:101-352 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT33973 NZ_AP021965:101-352 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References