33969

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4499151..4499648 Replicon chromosome
Accession NZ_AP021963
Organism Escherichia coli strain WP3-W18-CRE-03

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H7R02_RS21780 Protein ID WP_001173343.1
Coordinates 4499151..4499324 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag H7R02_RS21785 Protein ID WP_001312331.1
Coordinates 4499352..4499648 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R02_RS21770 4495821..4497278 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
H7R02_RS21775 4497515..4499032 + 1518 WP_000003806.1 lysine--tRNA ligase -
H7R02_RS21780 4499151..4499324 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H7R02_RS21785 4499352..4499648 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H7R02_RS21790 4499877..4500149 - 273 WP_000405647.1 N-acetyltransferase -
H7R02_RS21795 4500161..4500391 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
H7R02_RS21800 4500572..4502203 + 1632 WP_001216482.1 sensor histidine kinase -
H7R02_RS21805 4502200..4502919 + 720 WP_001540474.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4430423..4499648 69225


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T33969 WP_001173343.1 NZ_AP021963:c4499324-4499151 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T33969 NZ_AP021963:c4499324-4499151 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT33969 WP_001312331.1 NZ_AP021963:c4499648-4499352 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT33969 NZ_AP021963:c4499648-4499352 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References