Detailed information of TA system TA033967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4228584..4229398
• Replicon: chromosome
• Accession: NZ_AP021963
• Organism: Escherichia coli strain WP3-W18-CRE-03

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: H7R02_RS20420
• Protein ID: WP_001054376.1
• Coordinates: 4228584..4228841 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: S1NWL7
• Locus tag: H7R02_RS20425
• Protein ID: WP_001540600.1
• Coordinates: 4228853..4229398 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R02_RS20400	4223872..4224978	+	1107	WP_001774143.1	N-acetylneuraminate epimerase	-
H7R02_RS20405	4225043..4226023	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
H7R02_RS20410	4226606..4227846	-	1241	Protein_3997	DNA helicase	-
H7R02_RS20415	4227962..4228207	+	246	Protein_3998	GNAT family N-acetyltransferase	-
H7R02_RS20420	4228584..4228841	+	258	WP_001054376.1	hypothetical protein	Toxin
H7R02_RS20425	4228853..4229398	+	546	WP_001540600.1	N-acetyltransferase	Antitoxin
H7R02_RS20430	4229454..4230200	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
H7R02_RS20435	4230369..4230587	+	219	Protein_4002	hypothetical protein	-
H7R02_RS25360	4230625..4230741	+	117	Protein_4003	VOC family protein	-
H7R02_RS20440	4230986..4232107	+	1122	WP_000010829.1	M42 family metallopeptidase	-
H7R02_RS20445	4232104..4232382	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
H7R02_RS20450	4232394..4233707	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4218370..4237622	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T33967 WP_001054376.1 NZ_AP021963:4228584-4228841 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T33967 NZ_AP021963:4228584-4228841 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19926.87 Da
• Isoelectric Point: 6.3277

>AT33967 WP_001540600.1 NZ_AP021963:4228853-4229398 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFGKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT33967 NZ_AP021963:4228853-4229398 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCGGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: S1NWL7