Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4074875..4075425 Replicon chromosome
Accession NZ_AP021963
Organism Escherichia coli strain WP3-W18-CRE-03

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag H7R02_RS19680 Protein ID WP_001160966.1
Coordinates 4074875..4075189 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag H7R02_RS19685 Protein ID WP_000125568.1
Coordinates 4075192..4075425 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R02_RS19655 4070550..4071836 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
H7R02_RS19660 4071836..4072825 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H7R02_RS19665 4072822..4073643 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H7R02_RS19670 4073646..4074023 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H7R02_RS19675 4074030..4074878 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H7R02_RS19680 4074875..4075189 - 315 WP_001160966.1 CcdB family protein Toxin
H7R02_RS19685 4075192..4075425 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H7R02_RS19690 4075511..4075990 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
H7R02_RS19695 4076182..4078044 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
H7R02_RS19700 4078037..4078567 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H7R02_RS19705 4078675..4080006 - 1332 WP_001183207.1 MFS transporter -
H7R02_RS19710 4080065..4080352 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T33960 WP_001160966.1 NZ_AP021963:c4075189-4074875 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T33960 NZ_AP021963:c4075189-4074875 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT33960 WP_000125568.1 NZ_AP021963:c4075425-4075192 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT33960 NZ_AP021963:c4075425-4075192 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAACCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References