Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20563..21088 Replicon plasmid pWP3-W18-CRE-02_5
Accession NZ_AP021955
Organism Klebsiella quasipneumoniae strain WP3-W18-CRE-02

Toxin (Protein)


Gene name ccdB Uniprot ID A0A9E1GGX2
Locus tag H7R86_RS30325 Protein ID WP_016946795.1
Coordinates 20563..20868 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A9E1GDP8
Locus tag H7R86_RS30330 Protein ID WP_004197642.1
Coordinates 20870..21088 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R86_RS30295 16282..16908 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
H7R86_RS30300 16905..17207 + 303 WP_071571079.1 hypothetical protein -
H7R86_RS30305 17639..18433 - 795 WP_004197635.1 site-specific integrase -
H7R86_RS30310 18631..19647 - 1017 WP_017899884.1 hypothetical protein -
H7R86_RS30315 19658..19972 - 315 WP_053389906.1 hypothetical protein -
H7R86_RS30320 19999..20394 - 396 WP_017899885.1 hypothetical protein -
H7R86_RS30325 20563..20868 - 306 WP_016946795.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R86_RS30330 20870..21088 - 219 WP_004197642.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R86_RS30335 21306..21503 - 198 Protein_24 hypothetical protein -
H7R86_RS30340 21682..22656 - 975 WP_009309924.1 sensor domain-containing diguanylate cyclase -
H7R86_RS30345 22887..23903 - 1017 WP_182980895.1 IS5-like element IS5 family transposase -
H7R86_RS30350 23937..24329 + 393 Protein_27 IS630 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..91316 91316
- flank IS/Tn - - 22887..23867 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11548.22 Da        Isoelectric Point: 5.6831

>T33941 WP_016946795.1 NZ_AP021955:c20868-20563 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T33941 NZ_AP021955:c20868-20563 [Klebsiella quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8376.34 Da        Isoelectric Point: 4.7405

>AT33941 WP_004197642.1 NZ_AP021955:c21088-20870 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT33941 NZ_AP021955:c21088-20870 [Klebsiella quasipneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGAATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References