Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 165013..165749 Replicon plasmid pWP3-W18-CRE-02_2
Accession NZ_AP021952
Organism Klebsiella quasipneumoniae strain WP3-W18-CRE-02

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag H7R86_RS28620 Protein ID WP_003026803.1
Coordinates 165013..165495 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H7R86_RS28625 Protein ID WP_003026799.1
Coordinates 165483..165749 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R86_RS28600 160723..161127 - 405 WP_064145647.1 helix-turn-helix domain-containing protein -
H7R86_RS28605 161176..162522 - 1347 WP_182972103.1 ISNCY family transposase -
H7R86_RS28610 162748..163380 + 633 WP_001567369.1 hypothetical protein -
H7R86_RS28615 163409..164812 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
H7R86_RS28620 165013..165495 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
H7R86_RS28625 165483..165749 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H7R86_RS28630 166194..166424 - 231 WP_004206660.1 hypothetical protein -
H7R86_RS28635 166438..166641 - 204 WP_004206661.1 hemolysin expression modulator Hha -
H7R86_RS28640 166702..167193 - 492 WP_182980880.1 DNA-binding protein -
H7R86_RS28645 167327..168538 - 1212 WP_182972058.1 hypothetical protein -
H7R86_RS28650 168535..168783 - 249 WP_004193994.1 hypothetical protein -
H7R86_RS28655 169141..169491 + 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
H7R86_RS28660 169543..169905 + 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Il / blaGES-4 / catB8 / aac(6')-31 / qacE / sul1 - 1..206811 206811


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T33940 WP_003026803.1 NZ_AP021952:c165495-165013 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T33940 NZ_AP021952:c165495-165013 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT33940 WP_003026799.1 NZ_AP021952:c165749-165483 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT33940 NZ_AP021952:c165749-165483 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References