Detailed information of TA system TA033937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 222006..222757
• Replicon: plasmid pWP3-W18-CRE-02_1
• Accession: NZ_AP021951
• Organism: Klebsiella quasipneumoniae strain WP3-W18-CRE-02

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: H7R86_RS27515
• Protein ID: WP_101826042.1
• Coordinates: 222006..222488 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A6S4YWG5
• Locus tag: H7R86_RS27520
• Protein ID: WP_012540170.1
• Coordinates: 222479..222757 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R86_RS27490	218029..218757	-	729	WP_182952511.1	hypothetical protein	-
H7R86_RS27495	219802..220038	+	237	WP_012540047.1	hypothetical protein	-
H7R86_RS27500	220133..220330	-	198	WP_109037821.1	bacteriocin immunity protein	-
H7R86_RS27505	220394..221227	-	834	WP_012540133.1	GIY-YIG nuclease family protein	-
H7R86_RS27510	221564..221968	-	405	WP_048293664.1	DUF2251 domain-containing protein	-
H7R86_RS27515	222006..222488	-	483	WP_101826042.1	GNAT family N-acetyltransferase	Toxin
H7R86_RS27520	222479..222757	-	279	WP_012540170.1	DUF1778 domain-containing protein	Antitoxin
H7R86_RS27525	223065..223601	+	537	WP_182952510.1	hypothetical protein	-
H7R86_RS27530	224605..224919	+	315	WP_017146616.1	hypothetical protein	-
H7R86_RS27535	225163..225585	-	423	WP_182952522.1	hypothetical protein	-
H7R86_RS27540	225592..226191	-	600	WP_123282530.1	recombinase family protein	-
H7R86_RS27545	226626..227630	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..267818	267818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17698.59 Da
• Isoelectric Point: 9.1776

>T33937 WP_101826042.1 NZ_AP021951:c222488-222006 [Klebsiella quasipneumoniae]
MGMRPPEPLTPEHNIADFCCQDQVLSEWLKKKALKNHSTGLSRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTEHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Nucleotide

• Length: 483 bp

>T33937 NZ_AP021951:c222488-222006 [Klebsiella quasipneumoniae]
ATGGGAATGAGGCCTCCGGAACCTCTTACCCCTGAACATAACATTGCTGATTTTTGTTGTCAGGATCAGGTTCTTTCTGA
GTGGTTGAAGAAAAAGGCGCTGAAGAACCATAGCACGGGCCTTTCAAGAGTTTTCGTCGTCTGTGCTGAAAATACGAATA
GGGTAATTGCGTACTACTGTTTGGCATCCGGAAGCGTGCACAGAAACACCGTACCCGGAGCATACCGTCGTAATGCACCA
GAGGCTTTACCGGTCATTGTTCTGGGAAGGCTTGCGGTTGACGCTGCCTGGGCAAGAAAAGGGCTGGGAGCTGCACTGCT
TAAGGATGCGATATACCGAACAGAACATATTGCAATACAGGTTGGTGTTAGGGCGCTTCTTGTTCACGCCTTAAATGATG
AAGTTAGGGAATTTTACACAAAGTTTGGATTCGAGCCCTCCATTGCCAATGCGCTGACATTATTATTTCCGATCAAAACT
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10749.33 Da
• Isoelectric Point: 4.6722

>AT33937 WP_012540170.1 NZ_AP021951:c222757-222479 [Klebsiella quasipneumoniae]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFNAFVEELDAPMTPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT33937 NZ_AP021951:c222757-222479 [Klebsiella quasipneumoniae]
ATGAAACCAGACATTCGTGAGGCTCCGATAAACATCCGGGCGAAAACATCTCAACGCGATCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTGGAAGTGGCCTGCCGCGAAGCGCAGGACATCTTGTTAGATCAGCGCT
TGTTTCTGCTGGATGATAAGCAGTTTAACGCGTTTGTCGAAGAGCTTGACGCCCCTATGACCCCAGAGCGTCAGGCGCGT
ATTGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6S4YWG5