Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4080322..4080941 Replicon chromosome
Accession NZ_AP021950
Organism Klebsiella quasipneumoniae strain WP3-W18-CRE-02

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H7R86_RS20165 Protein ID WP_002892050.1
Coordinates 4080723..4080941 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag H7R86_RS20160 Protein ID WP_002892066.1
Coordinates 4080322..4080696 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R86_RS20150 4075479..4076672 + 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7R86_RS20155 4076695..4079841 + 3147 WP_004204748.1 multidrug efflux RND transporter permease subunit -
H7R86_RS20160 4080322..4080696 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
H7R86_RS20165 4080723..4080941 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H7R86_RS20170 4081099..4081665 + 567 WP_004204750.1 maltose O-acetyltransferase -
H7R86_RS20175 4081637..4081759 - 123 WP_032426076.1 hypothetical protein -
H7R86_RS20180 4081802..4082272 + 471 WP_182971413.1 YlaC family protein -
H7R86_RS20185 4082241..4083698 - 1458 WP_182971414.1 PLP-dependent aminotransferase family protein -
H7R86_RS20190 4083799..4084497 + 699 WP_004204753.1 GNAT family N-acetyltransferase -
H7R86_RS20195 4084494..4084634 - 141 WP_182972050.1 type B 50S ribosomal protein L36 -
H7R86_RS20200 4084634..4084897 - 264 WP_004204754.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T33934 WP_002892050.1 NZ_AP021950:4080723-4080941 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T33934 NZ_AP021950:4080723-4080941 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGCCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT33934 WP_002892066.1 NZ_AP021950:4080322-4080696 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT33934 NZ_AP021950:4080322-4080696 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References