Detailed information of TA system TA033918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 48207..48766
• Replicon: plasmid pWP2-S18-ESBL-08_2
• Accession: NZ_AP021948
• Organism: Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: H7R07_RS26315
• Protein ID: WP_000421259.1
• Coordinates: 48491..48766 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: H7R07_RS26310
• Protein ID: WP_001178087.1
• Coordinates: 48207..48491 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R07_RS26290	43856..44389	-	534	WP_001372168.1	transcription termination factor NusG	-
H7R07_RS26295	44831..45118	-	288	WP_000074849.1	conjugal transfer protein TraA	-
H7R07_RS26300	45036..45299	-	264	WP_001324596.1	ash family protein	-
H7R07_RS26305	46272..47303	+	1032	WP_000907852.1	replication initiation protein	-
H7R07_RS26310	48207..48491	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R07_RS26315	48491..48766	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R07_RS26320	48872..49153	+	282	WP_001105066.1	hypothetical protein	-
H7R07_RS26325	49345..49611	-	267	WP_000627832.1	hypothetical protein	-
H7R07_RS26330	49611..50105	-	495	WP_015058884.1	hypothetical protein	-
H7R07_RS26335	50161..50763	-	603	WP_061671620.1	proQ/FINO family protein	-
H7R07_RS26340	51117..52463	+	1347	WP_001132027.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-8	-	1..91630	91630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T33918 WP_000421259.1 NZ_AP021948:48491-48766 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T33918 NZ_AP021948:48491-48766 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT33918 WP_001178087.1 NZ_AP021948:48207-48491 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT33918 NZ_AP021948:48207-48491 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625