Detailed information of TA system TA033917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 196845..197221
• Replicon: plasmid pWP2-S18-ESBL-08_1
• Accession: NZ_AP021947
• Organism: Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: H7R07_RS26030
• Protein ID: WP_000969988.1
• Coordinates: 197039..197221 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: H7R07_RS26025
• Protein ID: WP_001324224.1
• Coordinates: 196845..197042 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R07_RS26005	192105..192341	+	237	WP_001323890.1	colicin V immunity protein	-
H7R07_RS26010	192319..192630	+	312	WP_001259758.1	colicin V	-
H7R07_RS26015	192800..194914	-	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
H7R07_RS26020	194889..196163	-	1275	WP_000489608.1	HlyD family secretion protein	-
H7R07_RS26025	196845..197042	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
H7R07_RS26030	197039..197221	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R07_RS26035	197320..197628	+	309	WP_000377483.1	hypothetical protein	-
H7R07_RS26040	198188..199222	+	1035	WP_001190233.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB	1..210712	210712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T33917 WP_000969988.1 NZ_AP021947:197039-197221 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T33917 NZ_AP021947:197039-197221 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT33917 WP_001324224.1 NZ_AP021947:196845-197042 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT33917 NZ_AP021947:196845-197042 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3