Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52519..53044 Replicon plasmid pWP2-S18-ESBL-08_1
Accession NZ_AP021947
Organism Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H7R07_RS25210 Protein ID WP_001159871.1
Coordinates 52739..53044 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag H7R07_RS25205 Protein ID WP_000813630.1
Coordinates 52519..52737 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R07_RS25180 48784..49329 + 546 WP_182913138.1 hypothetical protein -
H7R07_RS25185 49491..49907 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -
H7R07_RS25190 49904..50134 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R07_RS25195 50553..51242 + 690 WP_001513524.1 RES family NAD+ phosphorylase -
H7R07_RS25200 51274..51963 - 690 WP_001513525.1 helix-turn-helix domain-containing protein -
H7R07_RS25205 52519..52737 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R07_RS25210 52739..53044 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R07_RS25215 53045..53827 + 783 WP_001513526.1 site-specific integrase -
H7R07_RS25220 53929..55142 + 1214 WP_174245762.1 IS3 family transposase -
H7R07_RS25225 55840..56013 + 174 Protein_54 RepB family plasmid replication initiator protein -
H7R07_RS25235 57409..57855 + 447 WP_053270998.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB 1..210712 210712


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T33906 WP_001159871.1 NZ_AP021947:52739-53044 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T33906 NZ_AP021947:52739-53044 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT33906 WP_000813630.1 NZ_AP021947:52519-52737 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT33906 NZ_AP021947:52519-52737 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References