Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 49491..50134 Replicon plasmid pWP2-S18-ESBL-08_1
Accession NZ_AP021947
Organism Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag H7R07_RS25185 Protein ID WP_001044768.1
Coordinates 49491..49907 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag H7R07_RS25190 Protein ID WP_001261287.1
Coordinates 49904..50134 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R07_RS25160 44522..46087 - 1566 WP_001128474.1 AAA family ATPase -
H7R07_RS25165 46162..46578 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H7R07_RS25170 46575..46805 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R07_RS25175 47192..48526 + 1335 WP_182913135.1 hypothetical protein -
H7R07_RS25180 48784..49329 + 546 WP_182913138.1 hypothetical protein -
H7R07_RS25185 49491..49907 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R07_RS25190 49904..50134 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R07_RS25195 50553..51242 + 690 WP_001513524.1 RES family NAD+ phosphorylase -
H7R07_RS25200 51274..51963 - 690 WP_001513525.1 helix-turn-helix domain-containing protein -
H7R07_RS25205 52519..52737 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
H7R07_RS25210 52739..53044 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
H7R07_RS25215 53045..53827 + 783 WP_001513526.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB 1..210712 210712


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T33905 WP_001044768.1 NZ_AP021947:c49907-49491 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T33905 NZ_AP021947:c49907-49491 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGATGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTGACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT33905 WP_001261287.1 NZ_AP021947:c50134-49904 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT33905 NZ_AP021947:c50134-49904 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGCTCATTCCTGGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References