33872

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 23888..24531 Replicon plasmid pWP2-S18-ESBL-07_1
Accession NZ_AP021945
Organism Escherichia coli strain WP2-S18-ESBL-07

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag H7R05_RS22810 Protein ID WP_001034044.1
Coordinates 23888..24304 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag H7R05_RS22815 Protein ID WP_001261286.1
Coordinates 24301..24531 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R05_RS22790 19211..20464 + 1254 Protein_18 DUF4158 domain-containing protein -
H7R05_RS22795 20520..21216 + 697 Protein_19 IS1 family transposase -
H7R05_RS22800 21241..22263 - 1023 WP_000361402.1 helicase UvrD -
H7R05_RS22805 22248..23813 - 1566 WP_001128474.1 AAA family ATPase -
H7R05_RS22810 23888..24304 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R05_RS22815 24301..24531 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R05_RS22820 25134..25448 + 315 WP_001302628.1 hypothetical protein -
H7R05_RS22825 25501..26280 + 780 WP_000535297.1 hypothetical protein -
H7R05_RS22830 26331..27110 + 780 WP_024139091.1 site-specific integrase -
H7R05_RS22835 27248..27523 - 276 WP_000239529.1 hypothetical protein -
H7R05_RS22840 27517..28161 - 645 WP_000633913.1 AAA family ATPase -
H7R05_RS22845 28390..29361 + 972 WP_001103690.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 - 1..103781 103781


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T33872 WP_001034044.1 NZ_AP021945:c24304-23888 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T33872 NZ_AP021945:c24304-23888 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTTGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT33872 WP_001261286.1 NZ_AP021945:c24531-24301 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT33872 NZ_AP021945:c24531-24301 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References