Detailed information of TA system TA033866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3545503..3546340
• Replicon: chromosome
• Accession: NZ_AP021944
• Organism: Escherichia coli strain WP2-S18-ESBL-07

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: H7R05_RS17125
• Protein ID: WP_000227784.1
• Coordinates: 3545798..3546340 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: H7R05_RS17120
• Protein ID: WP_001297137.1
• Coordinates: 3545503..3545814 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R05_RS17095	3540523..3541470	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
H7R05_RS17100	3541492..3543483	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
H7R05_RS17105	3543473..3544087	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
H7R05_RS17110	3544087..3544416	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
H7R05_RS17115	3544428..3545318	+	891	WP_000971336.1	heme o synthase	-
H7R05_RS17120	3545503..3545814	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
H7R05_RS17125	3545798..3546340	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
H7R05_RS17130	3546396..3547331	-	936	WP_001297127.1	sel1 repeat family protein	-
H7R05_RS17135	3547739..3549103	+	1365	WP_001000974.1	MFS transporter	-
H7R05_RS17140	3549231..3549722	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
H7R05_RS17145	3549890..3550801	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T33866 WP_000227784.1 NZ_AP021944:3545798-3546340 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T33866 NZ_AP021944:3545798-3546340 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT33866 WP_001297137.1 NZ_AP021944:3545503-3545814 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT33866 NZ_AP021944:3545503-3545814 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3