Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1062352..1062935 Replicon chromosome
Accession NZ_AP021944
Organism Escherichia coli strain WP2-S18-ESBL-07

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag H7R05_RS05100 Protein ID WP_000254745.1
Coordinates 1062600..1062935 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2J1DJJ7
Locus tag H7R05_RS05095 Protein ID WP_000581939.1
Coordinates 1062352..1062600 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R05_RS05080 1057474..1058634 - 1161 Protein_997 HAMP domain-containing protein -
H7R05_RS05085 1058691..1059992 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
H7R05_RS05090 1060040..1062274 + 2235 WP_000226815.1 GTP pyrophosphokinase -
H7R05_RS05095 1062352..1062600 + 249 WP_000581939.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
H7R05_RS05100 1062600..1062935 + 336 WP_000254745.1 endoribonuclease MazF Toxin
H7R05_RS05105 1063006..1063797 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
H7R05_RS05110 1064025..1065662 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
H7R05_RS05115 1065750..1067048 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

Antitoxin PemI-like

(2-82)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T33852 WP_000254745.1 NZ_AP021944:1062600-1062935 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T33852 NZ_AP021944:1062600-1062935 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9369.76 Da        Isoelectric Point: 4.4791

>AT33852 WP_000581939.1 NZ_AP021944:1062352-1062600 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT33852 NZ_AP021944:1062352-1062600 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGAGATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A2J1DJJ7

References