Detailed information of TA system TA033844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 3542..4091
• Replicon: plasmid pWP2-W18-ESBL-11_2
• Accession: NZ_AP021938
• Organism: Acinetobacter pittii strain WP2-W18-ESBL-11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9KJ78
• Locus tag: H7R23_RS20040
• Protein ID: WP_000176412.1
• Coordinates: 3542..3832 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9KCM7
• Locus tag: H7R23_RS20045
• Protein ID: WP_005140357.1
• Coordinates: 3816..4091 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R23_RS20015	101..1030	+	930	WP_182918118.1	replication initiation protein RepM	-
H7R23_RS20020	1109..1639	+	531	WP_017482483.1	plasmid replication DNA-binding protein	-
H7R23_RS20025	1988..2305	+	318	WP_005065053.1	hypothetical protein	-
H7R23_RS20030	2830..3351	+	522	WP_000999915.1	hypothetical protein	-
H7R23_RS20035	3348..3494	+	147	WP_000192351.1	hypothetical protein	-
H7R23_RS20040	3542..3832	-	291	WP_000176412.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H7R23_RS20045	3816..4091	-	276	WP_005140357.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H7R23_RS20050	4279..4518	-	240	WP_017482479.1	hypothetical protein	-
H7R23_RS20055	4755..5222	-	468	WP_017399702.1	DUF1643 domain-containing protein	-
H7R23_RS20060	5274..6365	-	1092	WP_017482473.1	HpaII family restriction endonuclease	-
H7R23_RS20065	6365..7447	-	1083	WP_017482472.1	DNA cytosine methyltransferase	-
H7R23_RS20070	7764..8648	-	885	WP_017482471.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10416	10416

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-96)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (9-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11187.12 Da
• Isoelectric Point: 6.9783

>T33844 WP_000176412.1 NZ_AP021938:c3832-3542 [Acinetobacter pittii]
MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTVPDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDN
NELILARIGSHSELFG

Nucleotide

• Length: 291 bp

>T33844 NZ_AP021938:c3832-3542 [Acinetobacter pittii]
ATGACACTGACGATTAGGTACAAGAAACAATTTAAAAAAGATTTTAAACGGGTAAGCTCAAACCCAAAGCTTAAGAAAGG
ACTCTTAATTTTGAAGGATCTTATTGATGAGCATTTGGTAAAAACAGGGACTGTTCCAGATGAGTATTTACCTCATCCTT
TGATTGGTGAGTGGAAACCCCATATGGAATGCCATATTTTACCTGATTTATTATTAATTTGGGATGTGGATGAAGATAAT
AATGAGCTAATACTTGCTCGGATTGGATCTCATTCTGAACTTTTTGGATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10290.65 Da
• Isoelectric Point: 4.6702

>AT33844 WP_005140357.1 NZ_AP021938:c4091-3816 [Acinetobacter pittii]
MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQVVNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQE
LFTDLEDDTDD

Nucleotide

• Length: 276 bp

>AT33844 NZ_AP021938:c4091-3816 [Acinetobacter pittii]
ATGAATACTTTGGCAAAAAAAGATGAGTATGTACGTGCTAGGGTTCCAAATGAATTAAAGGTTCAAGCGGAGCGAATTCT
AAAAGAAGTGGGTTTAAATACTACAGATGCTATTCGCTTATTATTAACGCAAGTAGTAAATCGAGGTGGATTTCCTTTAG
AGTTAAGAACCCCTAACCGTACAACAATTGAGGCTATGAACTCTGTTGCAGAACCTAAAAAATTTAGTTCTGCTCAGGAA
TTGTTTACGGATTTAGAAGATGACACTGACGATTAG

Structures

Toxin

• AlphaFold DB: N9KJ78

Antitoxin

• AlphaFold DB: N9KCM7