Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4337039..4337561 Replicon chromosome
Accession NZ_AP021935
Organism Escherichia coli strain WP2-W18-ESBL-10

Toxin (Protein)


Gene name relE Uniprot ID A0A829L6G8
Locus tag H7X62_RS20470 Protein ID WP_001105433.1
Coordinates 4337271..4337561 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag H7X62_RS20465 Protein ID WP_000212715.1
Coordinates 4337039..4337281 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7X62_RS20445 4332758..4334131 + 1374 WP_001522402.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -
H7X62_RS20450 4334281..4334832 - 552 WP_000166270.1 ribosome-associated protein -
H7X62_RS20455 4334926..4336278 + 1353 WP_001162173.1 metalloprotease PmbA -
H7X62_RS20460 4336461..4336847 + 387 WP_001232246.1 cytochrome b562 -
H7X62_RS20465 4337039..4337281 + 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
H7X62_RS20470 4337271..4337561 + 291 WP_001105433.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7X62_RS20475 4337562..4338026 - 465 WP_001009182.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
H7X62_RS20480 4338206..4340344 - 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
H7X62_RS20485 4340738..4342393 - 1656 WP_001550509.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11367.23 Da        Isoelectric Point: 10.0238

>T33839 WP_001105433.1 NZ_AP021935:4337271-4337561 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T33839 NZ_AP021935:4337271-4337561 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT33839 WP_000212715.1 NZ_AP021935:4337039-4337281 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT33839 NZ_AP021935:4337039-4337281 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L6G8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References