Detailed information of TA system TA033821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1660430..1661135
• Replicon: chromosome
• Accession: NZ_AP021935
• Organism: Escherichia coli strain WP2-W18-ESBL-10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: H7X62_RS07725
• Protein ID: WP_000539521.1
• Coordinates: 1660749..1661135 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H7X62_RS07720
• Protein ID: WP_001280945.1
• Coordinates: 1660430..1660759 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7X62_RS07705	1655587..1656498	+	912	Protein_1498	glutathione ABC transporter permease GsiD	-
H7X62_RS07710	1656676..1659024	+	2349	WP_001550886.1	EAL domain-containing protein	-
H7X62_RS07715	1659032..1660360	+	1329	WP_001550887.1	GGDEF domain-containing protein	-
H7X62_RS07720	1660430..1660759	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
H7X62_RS07725	1660749..1661135	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7X62_RS07730	1661361..1662686	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
H7X62_RS07735	1662899..1663282	+	384	WP_000497137.1	biofilm formation regulator BssR	-
H7X62_RS07740	1663393..1664508	+	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
H7X62_RS07745	1664505..1665131	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T33821 WP_000539521.1 NZ_AP021935:c1661135-1660749 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T33821 NZ_AP021935:c1661135-1660749 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT33821 WP_001280945.1 NZ_AP021935:c1660759-1660430 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT33821 NZ_AP021935:c1660759-1660430 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6