Detailed information of TA system TA033817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 691428..691936
• Replicon: chromosome
• Accession: NZ_AP021935
• Organism: Escherichia coli strain WP2-W18-ESBL-10

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: H7X62_RS03290
• Protein ID: WP_001534843.1
• Coordinates: 691428..691637 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: H7X62_RS03295
• Protein ID: WP_000063161.1
• Coordinates: 691634..691936 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7X62_RS03275	687183..688493	-	1311	WP_001534837.1	restriction endonuclease subunit S	-
H7X62_RS03280	688483..690102	-	1620	WP_001550561.1	type I restriction-modification system subunit M	-
H7X62_RS03285	690166..691332	-	1167	WP_001534841.1	restriction endonuclease	-
H7X62_RS03290	691428..691637	+	210	WP_001534843.1	BrnT family toxin	Toxin
H7X62_RS03295	691634..691936	+	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
H7X62_RS03300	691970..692926	-	957	WP_001534845.1	GTPase	-
H7X62_RS03305	692937..693140	-	204	WP_001534846.1	YbdD/YjiX family protein	-
H7X62_RS03310	693258..695408	-	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T33817 WP_001534843.1 NZ_AP021935:691428-691637 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T33817 NZ_AP021935:691428-691637 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT33817 WP_000063161.1 NZ_AP021935:691634-691936 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT33817 NZ_AP021935:691634-691936 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3