Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4245730..4246252 Replicon chromosome
Accession NZ_AP021933
Organism Escherichia coli strain WP2-W18-ESBL-08

Toxin (Protein)


Gene name relE Uniprot ID V0SDB6
Locus tag H7R10_RS20065 Protein ID WP_001105432.1
Coordinates 4245730..4246020 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag H7R10_RS20070 Protein ID WP_000212715.1
Coordinates 4246010..4246252 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R10_RS20050 4240893..4242548 + 1656 WP_001361372.1 alpha,alpha-phosphotrehalase -
H7R10_RS20055 4242942..4245080 + 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
H7R10_RS20060 4245265..4245729 + 465 WP_001009180.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
H7R10_RS20065 4245730..4246020 - 291 WP_001105432.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R10_RS20070 4246010..4246252 - 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
H7R10_RS20075 4246444..4246830 - 387 WP_001232253.1 cytochrome b562 -
H7R10_RS20080 4246886..4248238 - 1353 WP_001162172.1 metalloprotease PmbA -
H7R10_RS20085 4248332..4248883 + 552 WP_000166270.1 ribosome-associated protein -
H7R10_RS20090 4248966..4250339 - 1374 WP_001219814.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11311.21 Da        Isoelectric Point: 10.0238

>T33802 WP_001105432.1 NZ_AP021933:c4246020-4245730 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTLGYRLVYSVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T33802 NZ_AP021933:c4246020-4245730 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT33802 WP_000212715.1 NZ_AP021933:c4246252-4246010 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT33802 NZ_AP021933:c4246252-4246010 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SDB6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References