Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4150862..4151438 Replicon chromosome
Accession NZ_AP021933
Organism Escherichia coli strain WP2-W18-ESBL-08

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag H7R10_RS19645 Protein ID WP_001131965.1
Coordinates 4151151..4151438 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag H7R10_RS19640 Protein ID WP_000063158.1
Coordinates 4150862..4151164 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R10_RS19620 4145970..4147190 + 1221 WP_000873756.1 amino acid deaminase -
H7R10_RS19625 4147458..4149608 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
H7R10_RS19630 4149658..4149861 + 204 WP_000467859.1 YbdD/YjiX family protein -
H7R10_RS19635 4149872..4150828 + 957 WP_001361361.1 GTPase -
H7R10_RS19640 4150862..4151164 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
H7R10_RS19645 4151151..4151438 - 288 WP_001131965.1 BrnT family toxin Toxin
H7R10_RS19650 4151635..4152801 + 1167 WP_000800823.1 restriction endonuclease -
H7R10_RS19655 4152846..4153019 + 174 Protein_3847 SAM-dependent DNA methyltransferase -
H7R10_RS19660 4153013..4153681 + 669 Protein_3848 DUF3387 domain-containing protein -
H7R10_RS19665 4153896..4154234 + 339 WP_000132633.1 endoribonuclease SymE -
H7R10_RS19670 4154276..4154440 - 165 WP_000394263.1 DUF1127 domain-containing protein -
H7R10_RS19675 4154617..4156029 + 1413 WP_113248463.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T33801 WP_001131965.1 NZ_AP021933:c4151438-4151151 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T33801 NZ_AP021933:c4151438-4151151 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT33801 WP_000063158.1 NZ_AP021933:c4151164-4150862 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT33801 NZ_AP021933:c4151164-4150862 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References