Detailed information of TA system TA033800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3651676..3652513
• Replicon: chromosome
• Accession: NZ_AP021933
• Organism: Escherichia coli strain WP2-W18-ESBL-08

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A1M0D5U3
• Locus tag: H7R10_RS17265
• Protein ID: WP_000227782.1
• Coordinates: 3651971..3652513 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: H7R10_RS17260
• Protein ID: WP_001353405.1
• Coordinates: 3651676..3651987 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R10_RS17235	3646696..3647643	+	948	WP_001239437.1	cytochrome o ubiquinol oxidase subunit II	-
H7R10_RS17240	3647665..3649656	+	1992	WP_182913424.1	cytochrome o ubiquinol oxidase subunit I	-
H7R10_RS17245	3649646..3650260	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
H7R10_RS17250	3650260..3650589	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
H7R10_RS17255	3650601..3651491	+	891	WP_000971327.1	heme o synthase	-
H7R10_RS17260	3651676..3651987	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
H7R10_RS17265	3651971..3652513	+	543	WP_000227782.1	GNAT family N-acetyltransferase	Toxin
H7R10_RS17270	3652569..3653413	-	845	Protein_3381	sel1 repeat family protein	-
H7R10_RS17275	3653820..3655184	+	1365	WP_001000978.1	MFS transporter	-
H7R10_RS17280	3655312..3655803	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
H7R10_RS17285	3655971..3656882	+	912	WP_000705841.1	2-dehydropantoate 2-reductase	-
H7R10_RS17290	3656845..3657435	+	591	WP_001276320.1	protein deglycase YajL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19957.31 Da
• Isoelectric Point: 9.1763

>T33800 WP_000227782.1 NZ_AP021933:3651971-3652513 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T33800 NZ_AP021933:3651971-3652513 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTATTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTAATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT33800 WP_001353405.1 NZ_AP021933:3651676-3651987 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT33800 NZ_AP021933:3651676-3651987 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A1M0D5U3

Antitoxin

• AlphaFold DB: A0A829G7G3