Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3617586..3618204 Replicon chromosome
Accession NZ_AP021933
Organism Escherichia coli strain WP2-W18-ESBL-08

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H7R10_RS17095 Protein ID WP_001291435.1
Coordinates 3617986..3618204 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H7R10_RS17090 Protein ID WP_000344800.1
Coordinates 3617586..3617960 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R10_RS17080 3612675..3613868 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7R10_RS17085 3613891..3617040 + 3150 WP_113248486.1 efflux RND transporter permease AcrB -
H7R10_RS17090 3617586..3617960 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H7R10_RS17095 3617986..3618204 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H7R10_RS17100 3618376..3618927 + 552 WP_000102574.1 maltose O-acetyltransferase -
H7R10_RS17105 3619043..3619513 + 471 WP_000136192.1 YlaC family protein -
H7R10_RS17110 3619677..3621227 + 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H7R10_RS17115 3621269..3621622 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H7R10_RS17125 3622001..3622312 + 312 WP_000409911.1 MGMT family protein -
H7R10_RS17130 3622343..3622915 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T33799 WP_001291435.1 NZ_AP021933:3617986-3618204 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T33799 NZ_AP021933:3617986-3618204 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT33799 WP_000344800.1 NZ_AP021933:3617586-3617960 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT33799 NZ_AP021933:3617586-3617960 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References