Detailed information of TA system TA033797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3187515..3188220
• Replicon: chromosome
• Accession: NZ_AP021933
• Organism: Escherichia coli strain WP2-W18-ESBL-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: H7R10_RS14945
• Protein ID: WP_000539524.1
• Coordinates: 3187515..3187901 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H7R10_RS14950
• Protein ID: WP_001280945.1
• Coordinates: 3187891..3188220 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R10_RS14925	3183519..3184145	+	627	WP_001295292.1	glutathione S-transferase GstB	-
H7R10_RS14930	3184142..3185257	-	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
H7R10_RS14935	3185368..3185751	-	384	WP_000497137.1	biofilm formation regulator BssR	-
H7R10_RS14940	3185964..3187289	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
H7R10_RS14945	3187515..3187901	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R10_RS14950	3187891..3188220	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
H7R10_RS14955	3188290..3189618	-	1329	WP_000086870.1	GGDEF domain-containing protein	-
H7R10_RS14960	3189626..3191974	-	2349	WP_000950331.1	EAL domain-containing protein	-
H7R10_RS14965	3192151..3193062	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T33797 WP_000539524.1 NZ_AP021933:3187515-3187901 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T33797 NZ_AP021933:3187515-3187901 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAAGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAGGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT33797 WP_001280945.1 NZ_AP021933:3187891-3188220 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT33797 NZ_AP021933:3187891-3188220 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7