Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 166497..167109 Replicon chromosome
Accession NZ_AP021933
Organism Escherichia coli strain WP2-W18-ESBL-08

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag H7R10_RS00715 Protein ID WP_000833473.1
Coordinates 166497..166682 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1M2IVK0
Locus tag H7R10_RS00720 Protein ID WP_000499742.1
Coordinates 166699..167109 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R10_RS00700 161963..163258 + 1296 WP_000985745.1 Fic family protein -
H7R10_RS00705 163366..164904 + 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
H7R10_RS00710 164945..166024 - 1080 WP_000061475.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
H7R10_RS00715 166497..166682 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
H7R10_RS00720 166699..167109 + 411 WP_000499742.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H7R10_RS00725 167221..169200 - 1980 WP_001026863.1 glycoside hydrolase family 127 protein -
H7R10_RS00730 169211..170611 - 1401 WP_000204814.1 MFS transporter -
H7R10_RS00735 170837..171652 + 816 WP_000891820.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T33780 WP_000833473.1 NZ_AP021933:166497-166682 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T33780 NZ_AP021933:166497-166682 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15242.13 Da        Isoelectric Point: 4.4482

>AT33780 WP_000499742.1 NZ_AP021933:166699-167109 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT33780 NZ_AP021933:166699-167109 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGG
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IVK0

References