Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 101567..102303 Replicon plasmid pWP2-W18-ESBL-06_1
Accession NZ_AP021930
Organism Klebsiella pneumoniae strain WP2-W18-ESBL-06

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag H7R50_RS25420 Protein ID WP_003026803.1
Coordinates 101821..102303 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H7R50_RS25415 Protein ID WP_003026799.1
Coordinates 101567..101833 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R50_RS25400 96914..97492 - 579 WP_102084884.1 recombinase family protein -
H7R50_RS25405 97651..99943 + 2293 Protein_112 Tn3 family transposase -
H7R50_RS25410 100022..101026 + 1005 WP_000427619.1 IS110-like element IS5075 family transposase -
H7R50_RS25415 101567..101833 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H7R50_RS25420 101821..102303 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
H7R50_RS25425 102444..102776 + 333 Protein_116 ISNCY family transposase -
H7R50_RS25430 102936..103640 + 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -
H7R50_RS25435 103975..104232 - 258 WP_023292103.1 hypothetical protein -
H7R50_RS25440 104837..106291 + 1455 WP_182924419.1 EAL domain-containing protein -
H7R50_RS25445 106472..106771 + 300 WP_020325013.1 hypothetical protein -
H7R50_RS25450 106772..107086 + 315 WP_020325011.1 hypothetical protein -
H7R50_RS25455 107146..107274 + 129 Protein_122 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..140912 140912


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T33776 WP_003026803.1 NZ_AP021930:101821-102303 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T33776 NZ_AP021930:101821-102303 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT33776 WP_003026799.1 NZ_AP021930:101567-101833 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT33776 NZ_AP021930:101567-101833 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References