Detailed information of TA system TA033757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/DUF6088(antitoxin)
• Location: 83754..84357
• Replicon: plasmid pWP2-W18-ESBL-01_1
• Accession: NZ_AP021928
• Organism: Aeromonas caviae strain WP2-W18-ESBL-01

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: H7R60_RS22965
• Protein ID: WP_182936407.1
• Coordinates: 84142..84357 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A807ZF76
• Locus tag: H7R60_RS22960
• Protein ID: WP_042468273.1
• Coordinates: 83754..84152 (+)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R60_RS22940	79045..80181	+	1137	WP_182935169.1	ISAs1 family transposase	-
H7R60_RS22945	80411..81223	+	813	WP_182936405.1	ParA family protein	-
H7R60_RS22950	81229..81513	+	285	WP_182936406.1	hypothetical protein	-
H7R60_RS22955	81913..83166	+	1254	WP_042650430.1	IS256-like element ISAeme25 family transposase	-
H7R60_RS22960	83754..84152	+	399	WP_042468273.1	S-adenosylhomocysteine hydrolase	Antitoxin
H7R60_RS22965	84142..84357	+	216	WP_182936407.1	hypothetical protein	Toxin
H7R60_RS22970	84359..85612	-	1254	WP_042650430.1	IS256-like element ISAeme25 family transposase	-
H7R60_RS22975	85641..86069	+	429	WP_182936408.1	hypothetical protein	-
H7R60_RS22980	86109..87362	-	1254	WP_182936409.1	IS256 family transposase	-
H7R60_RS22985	87780..87932	+	153	WP_005321445.1	type I toxin-antitoxin system Hok family toxin	-
H7R60_RS22990	88062..88247	+	186	WP_005321442.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135252	135252
	-	inside	IScluster/Tn	-	-	72129..96071	23942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Protein of unknown function DUF6088 (7-128)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 8009.23 Da
• Isoelectric Point: 5.4560

>T33757 WP_182936407.1 NZ_AP021928:84142-84357 [Aeromonas caviae]
MQPKDSSIFADVSDYFGIDNPAIAEKDYYVVQLLKLLASHYCQHHTMVFAGGTALAKADVKLSYCQMWCME

Nucleotide

• Length: 216 bp

>T33757 NZ_AP021928:84142-84357 [Aeromonas caviae]
ATGCAGCCTAAAGACTCTTCGATTTTCGCCGACGTTTCTGACTACTTCGGGATCGATAACCCTGCGATAGCAGAAAAGGA
CTACTACGTCGTCCAGCTGCTCAAACTGCTTGCAAGTCATTATTGCCAGCACCACACCATGGTGTTTGCAGGCGGGACCG
CATTAGCCAAAGCTGACGTCAAGCTGAGCTATTGTCAAATGTGGTGTATGGAGTAG

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14761.36 Da
• Isoelectric Point: 11.0142

>AT33757 WP_042468273.1 NZ_AP021928:83754-84152 [Aeromonas caviae]
MTIADRVEARIKRSKRDVFLREDFDDLGGYDQIGRALRQMCEKGLLLKVGYGVYAKARRNRINSRLMIASPGGPDAVLIA
TLDRLKVPYELKGLTAAYAAGKSTQVPAFMEVKPKKRFSRKLKIGSRVFNAA

Nucleotide

• Length: 399 bp

>AT33757 NZ_AP021928:83754-84152 [Aeromonas caviae]
ATGACCATTGCTGACCGTGTTGAAGCACGAATCAAACGATCTAAGCGAGATGTGTTTCTCCGTGAGGATTTCGATGACTT
GGGCGGTTATGACCAAATAGGCAGAGCTTTACGCCAGATGTGTGAAAAGGGTCTGCTTCTGAAGGTCGGCTACGGCGTGT
ATGCAAAGGCACGGCGTAACCGGATCAATAGTCGCTTGATGATCGCCTCCCCTGGTGGTCCAGACGCCGTACTCATTGCT
ACGCTTGACCGCCTGAAGGTGCCTTACGAACTGAAGGGCCTGACCGCAGCTTATGCCGCGGGGAAATCCACTCAGGTTCC
CGCCTTTATGGAAGTTAAGCCTAAAAAGCGTTTTTCCAGAAAGCTGAAAATAGGAAGCCGAGTGTTCAATGCAGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A807ZF76