Detailed information of TA system TA033755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 13196..13752
• Replicon: plasmid pWP2-W18-ESBL-01_1
• Accession: NZ_AP021928
• Organism: Aeromonas caviae strain WP2-W18-ESBL-01

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H7R60_RS22645
• Protein ID: WP_182936448.1
• Coordinates: 13477..13752 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H7R60_RS22640
• Protein ID: WP_182936447.1
• Coordinates: 13196..13477 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R60_RS22615	8982..9272	+	291	WP_080871675.1	nucleotidyltransferase domain-containing protein	-
H7R60_RS22620	9269..9643	+	375	WP_080871676.1	DUF86 domain-containing protein	-
H7R60_RS22625	9637..10194	+	558	WP_080871677.1	recombinase family protein	-
H7R60_RS22630	10227..12419	-	2193	Protein_11	Tn3 family transposase	-
H7R60_RS22635	12423..12983	-	561	WP_182936446.1	recombinase family protein	-
H7R60_RS22640	13196..13477	+	282	WP_182936447.1	ribbon-helix-helix domain-containing protein	Antitoxin
H7R60_RS22645	13477..13752	+	276	WP_182936448.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R60_RS22650	13802..14530	+	729	WP_182936449.1	carbonic anhydrase	-
H7R60_RS22655	14904..16001	-	1098	WP_010676219.1	ISAs1 family transposase	-
H7R60_RS22660	16046..16411	+	366	WP_182936450.1	SLATT domain-containing protein	-
H7R60_RS22665	16615..17562	+	948	WP_012564931.1	IS30-like element ISAeca1 family transposase	-
H7R60_RS22670	17566..17928	-	363	WP_182936465.1	TIR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135252	135252
	-	inside	IScluster/Tn	-	-	5846..26917	21071

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10676.43 Da
• Isoelectric Point: 9.5291

>T33755 WP_182936448.1 NZ_AP021928:13477-13752 [Aeromonas caviae]
MELKWTSKALSDLTRLYEFLALANKPAAARVVQALTQAPAILLTNPRIGEQLFQFEPREVRRMLVGEYEVRYELLDSSIY
ILRLWHTREAR

Nucleotide

• Length: 276 bp

>T33755 NZ_AP021928:13477-13752 [Aeromonas caviae]
ATGGAACTGAAGTGGACCAGCAAAGCGCTCTCTGATTTGACACGCTTGTATGAATTTTTGGCTCTGGCTAACAAACCCGC
CGCGGCTCGGGTAGTGCAGGCACTGACACAGGCCCCAGCTATTCTGCTAACAAACCCGCGTATTGGAGAACAACTGTTCC
AATTCGAGCCCCGCGAGGTGAGGAGGATGTTGGTTGGAGAATACGAGGTTCGTTACGAGCTCCTTGATTCGAGCATCTAC
ATTCTGCGGCTGTGGCATACCCGAGAAGCTCGTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10368.83 Da
• Isoelectric Point: 5.6706

>AT33755 WP_182936447.1 NZ_AP021928:13196-13477 [Aeromonas caviae]
MKSNMTQATTKVLTAHVPLPMADKVDQMAARLERSRGWIMKQALSAWIAQEEDRDRLTQEALADVEAGRVIDHLAVQAWA
DSLSTDQPLSVPR

Nucleotide

• Length: 282 bp

>AT33755 NZ_AP021928:13196-13477 [Aeromonas caviae]
ATGAAGAGCAACATGACACAAGCAACAACCAAGGTGCTTACTGCACACGTACCACTACCTATGGCCGACAAAGTTGACCA
GATGGCAGCCCGCCTTGAACGCTCACGGGGCTGGATCATGAAACAGGCTCTTTCCGCATGGATCGCCCAGGAAGAGGATC
GTGATCGCCTGACCCAAGAGGCGCTGGCAGACGTGGAAGCTGGTCGAGTTATCGATCACTTGGCGGTGCAGGCTTGGGCT
GACAGCCTCAGTACTGACCAGCCGCTGTCGGTTCCGCGTTGA