Detailed information of TA system TA033754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2161840..2163404
• Replicon: chromosome
• Accession: NZ_AP021927
• Organism: Aeromonas caviae strain WP2-W18-ESBL-01

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A2S4TP05
• Locus tag: H7R60_RS10295
• Protein ID: WP_005329232.1
• Coordinates: 2161840..2163150 (-)
• Length: 437 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H7R60_RS10300
• Protein ID: WP_005329233.1
• Coordinates: 2163150..2163404 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R60_RS10280	2156875..2157993	-	1119	WP_197971249.1	DUF1214 domain-containing protein	-
H7R60_RS23335	2158201..2158392	-	192	WP_197971250.1	hypothetical protein	-
H7R60_RS10285	2158709..2160883	-	2175	WP_182936196.1	catalase/peroxidase HPI	-
H7R60_RS10295	2161840..2163150	-	1311	WP_005329232.1	type II toxin-antitoxin system HipA family toxin	Toxin
H7R60_RS10300	2163150..2163404	-	255	WP_005329233.1	helix-turn-helix domain-containing protein	Antitoxin
H7R60_RS10305	2163905..2164153	+	249	WP_010674978.1	hypothetical protein	-
H7R60_RS10310	2164574..2164825	-	252	WP_010674977.1	plasmid stabilization protein	-
H7R60_RS10315	2164904..2166286	-	1383	WP_197971251.1	Fic family protein	-
H7R60_RS10320	2166466..2166918	+	453	WP_021140686.1	DUF2384 domain-containing protein	-
H7R60_RS10325	2167250..2167828	+	579	WP_024941319.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 437 a.a.
• Molecular weight: 49096.31 Da
• Isoelectric Point: 7.1717

>T33754 WP_005329232.1 NZ_AP021927:c2163150-2161840 [Aeromonas caviae]
MASLTVYMNGYRVGTFTKATNGAHQFQYDESWVKLPGSRPISLSMPLRQQRYQGDEAYNFFDNLLPDNPEVRNRVVARYQ
ADSTQPFDLLSCIGQDSVGALQLVAQGRPVPDVKRIECKPLSDAELEQIMTSYQQGIPLGMVREEDDFRISIAGAQEKTA
LLYLDNRWCLPHAATPTTHIIKLPIGKIESHSYSIDLSQSVENEYLCTLIAKALGLPVPHCFIMQVGKVKALAVERFDRR
YATDRSWIMRLPQEDFCQVLNVPSARKYESHGGPGIADIMTTLLGSATPEQDRYLFMKSQVLFWLLAATDGHAKNFSVFI
EPEGRFRLTPFYDILSMYPAFGGKGLHPRDAKLAMGLTATKGKKYAIEQIFPRHFYQTAKAIGFEQVQMEKIISELTDNL
DEVISIVRQQLPDTFPTEIADSILGGLSSKAQRLKR

Nucleotide

• Length: 1311 bp

>T33754 NZ_AP021927:c2163150-2161840 [Aeromonas caviae]
ATGGCCAGCCTGACGGTCTACATGAACGGATACCGGGTAGGTACCTTTACCAAGGCGACCAATGGTGCTCACCAATTTCA
GTACGATGAATCGTGGGTGAAGTTGCCAGGCAGCCGCCCCATCTCGCTCTCCATGCCATTACGCCAGCAGCGCTACCAGG
GCGATGAGGCCTATAACTTCTTCGATAATCTGCTGCCAGATAACCCGGAAGTAAGAAACCGGGTGGTGGCGCGCTATCAA
GCCGATTCCACCCAACCCTTTGATCTGCTCAGTTGCATTGGCCAGGACAGCGTGGGGGCATTGCAACTGGTGGCTCAGGG
CAGACCTGTGCCAGACGTTAAGCGCATTGAGTGCAAGCCGCTCTCTGACGCCGAACTGGAGCAGATCATGACCAGCTACC
AGCAAGGGATCCCGCTTGGTATGGTGAGGGAAGAGGACGATTTTCGGATATCCATCGCCGGGGCTCAGGAAAAGACCGCG
TTACTCTACCTCGACAACCGTTGGTGCTTACCGCATGCAGCGACCCCAACCACCCACATTATCAAGCTGCCCATTGGCAA
GATCGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACCCTGATCGCCAAAGCAT
TGGGTCTTCCGGTTCCCCATTGCTTCATCATGCAGGTCGGCAAGGTCAAGGCTCTGGCGGTGGAACGCTTCGATCGGCGC
TACGCCACGGATCGCAGTTGGATCATGCGCCTGCCCCAAGAGGACTTCTGCCAGGTGTTGAATGTACCGTCAGCACGCAA
GTACGAGAGCCATGGTGGACCGGGCATTGCTGACATCATGACGACCCTGCTGGGATCGGCCACGCCGGAGCAGGATCGGT
ACCTGTTTATGAAGTCTCAGGTGTTGTTCTGGCTGCTGGCTGCCACTGATGGCCATGCCAAGAATTTCTCGGTGTTTATC
GAACCTGAAGGGCGTTTCCGGCTTACACCGTTTTACGACATCCTTTCCATGTATCCTGCATTTGGTGGTAAAGGATTACA
TCCCCGTGATGCCAAGTTGGCCATGGGGCTTACTGCAACCAAGGGCAAAAAATACGCGATAGAGCAGATATTTCCTCGCC
ATTTTTATCAAACGGCCAAGGCGATTGGTTTTGAACAAGTGCAGATGGAAAAAATCATCAGCGAACTGACGGATAATCTG
GATGAGGTAATATCAATCGTTCGTCAGCAATTGCCTGATACATTTCCCACTGAGATTGCAGATTCAATTTTGGGTGGACT
CAGTTCGAAAGCGCAACGCTTAAAACGATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9271.66 Da
• Isoelectric Point: 9.1571

>AT33754 WP_005329233.1 NZ_AP021927:c2163404-2163150 [Aeromonas caviae]
MKVTSAKQLSACLKDARLSQKLSQGKVAGKVGIRQDTVSSFELNPESTKLETLFKILAALELSLDIRPRTDPAENATPGW
KEEW

Nucleotide

• Length: 255 bp

>AT33754 NZ_AP021927:c2163404-2163150 [Aeromonas caviae]
ATGAAAGTAACCAGCGCCAAGCAACTTAGTGCCTGTCTCAAGGATGCCAGGCTTTCGCAAAAGCTCTCCCAAGGCAAAGT
GGCCGGCAAGGTGGGGATCAGGCAGGACACGGTGTCCAGCTTCGAGCTCAATCCCGAGTCAACCAAGCTGGAGACCTTGT
TCAAGATCCTCGCCGCACTGGAACTCTCCCTCGATATCAGGCCCCGCACCGATCCTGCGGAAAACGCCACGCCTGGCTGG
AAGGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A2S4TP05