Detailed information of TA system TA033751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4668..5236
• Replicon: plasmid pWP2-W18-CRE-05_6
• Accession: NZ_AP021923
• Organism: Aeromonas sp. WP2-W18-CRE-05

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: H7R73_RS23905
• Protein ID: WP_182951812.1
• Coordinates: 4943..5236 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3S7DUI6
• Locus tag: H7R73_RS23900
• Protein ID: WP_043849769.1
• Coordinates: 4668..4955 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R73_RS23875	194..1069	+	876	WP_182951807.1	replication initiation protein	-
H7R73_RS23880	1248..1538	-	291	WP_182951808.1	hypothetical protein	-
H7R73_RS23885	1751..2152	+	402	WP_182951809.1	plasmid mobilization relaxosome protein MobC	-
H7R73_RS23890	2142..3962	+	1821	WP_182951810.1	relaxase/mobilization nuclease domain-containing protein	-
H7R73_RS23895	4000..4287	+	288	WP_182951811.1	hypothetical protein	-
H7R73_RS23900	4668..4955	+	288	WP_043849769.1	CopG family ribbon-helix-helix protein	Antitoxin
H7R73_RS23905	4943..5236	+	294	WP_182951812.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R73_RS23910	5249..5545	+	297	WP_182951813.1	hypothetical protein	-
H7R73_RS23915	5646..6017	+	372	WP_182951814.1	type II toxin-antitoxin system CcdA family antitoxin	-
H7R73_RS23920	6230..6547	+	318	WP_032429244.1	multidrug efflux SMR transporter	-
H7R73_RS23925	6562..7851	+	1290	WP_023343400.1	ISL3 family transposase	-
H7R73_RS23930	7948..8373	+	426	WP_042938796.1	MarR family transcriptional regulator	-
H7R73_RS23935	8407..9732	+	1326	WP_065760461.1	MATE family efflux transporter	-
H7R73_RS23940	9757..10182	-	426	WP_042938792.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11369	11369
	-	flank	IS/Tn	-	-	6562..7851	1289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10906.71 Da
• Isoelectric Point: 10.8518

>T33751 WP_182951812.1 NZ_AP021923:4943-5236 [Aeromonas sp. WP2-W18-CRE-05]
MPQVILSPAALRDLKRLREFLHSKSPVVAQRAAQTIRSALTQLGQQPAMGRPIEGLPEVFREWVIPFGDSGYLARYRLDG
ETVVILALRHQREAGFI

Nucleotide

• Length: 294 bp

>T33751 NZ_AP021923:4943-5236 [Aeromonas sp. WP2-W18-CRE-05]
ATGCCACAAGTGATCCTCTCTCCGGCTGCTTTGCGTGACCTTAAACGGCTGCGCGAGTTCTTGCACAGTAAGAGCCCGGT
TGTTGCACAGCGAGCAGCCCAAACCATTCGTTCGGCGCTGACCCAGCTCGGGCAACAACCCGCGATGGGTCGTCCTATTG
AAGGGCTCCCGGAGGTGTTCCGCGAGTGGGTGATCCCCTTTGGGGACAGTGGTTATCTGGCGCGTTACCGCCTAGATGGC
GAAACCGTAGTGATCTTGGCTCTGCGTCACCAGCGCGAAGCCGGGTTTATTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10892.16 Da
• Isoelectric Point: 5.1897

>AT33751 WP_043849769.1 NZ_AP021923:4668-4955 [Aeromonas sp. WP2-W18-CRE-05]
MSAATKPMSIKLDSDTQSRIKQLAAARDRSAHWMMREAITQYVEREERKEAFRRDTLDAWQEFQETGLHVSLEEADQWLA
GWGTEIEGGAPACHK

Nucleotide

• Length: 288 bp

>AT33751 NZ_AP021923:4668-4955 [Aeromonas sp. WP2-W18-CRE-05]
ATGTCCGCAGCTACCAAACCCATGTCTATCAAACTTGATAGCGATACCCAAAGCCGGATCAAGCAGCTAGCTGCTGCCCG
CGACCGTTCAGCGCACTGGATGATGCGCGAAGCCATCACCCAGTATGTTGAGCGCGAAGAGCGCAAGGAGGCGTTCCGCC
GCGATACCCTCGATGCTTGGCAGGAGTTTCAGGAGACTGGTTTGCATGTCTCTCTTGAAGAGGCCGATCAGTGGTTGGCT
GGTTGGGGCACTGAAATTGAAGGTGGTGCACCTGCATGCCACAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DUI6