Detailed information of TA system TA033744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 124349..124725
• Replicon: plasmid pWP2-W18-CRE-03_1
• Accession: NZ_AP021915
• Organism: Escherichia coli strain WP2-W18-CRE-03

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: H7Q98_RS23495
• Protein ID: WP_000969988.1
• Coordinates: 124543..124725 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: H7Q98_RS23490
• Protein ID: WP_001324224.1
• Coordinates: 124349..124546 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7Q98_RS23470	119609..119845	+	237	WP_001323890.1	colicin V immunity protein	-
H7Q98_RS23475	119823..120134	+	312	WP_001259758.1	colicin V	-
H7Q98_RS23480	120304..122418	-	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
H7Q98_RS23485	122393..123667	-	1275	WP_000489609.1	HlyD family secretion protein	-
H7Q98_RS23490	124349..124546	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
H7Q98_RS23495	124543..124725	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7Q98_RS23500	124824..125132	+	309	WP_000377483.1	hypothetical protein	-
H7Q98_RS23505	125692..126726	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / tet(B) / blaCTX-M-27 / catA1 / oqxB / oqxA	iucA / iucB / iucC / iucD / iutA / iroN / iroE / iroD / iroC / iroB	1..158754	158754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T33744 WP_000969988.1 NZ_AP021915:124543-124725 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T33744 NZ_AP021915:124543-124725 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT33744 WP_001324224.1 NZ_AP021915:124349-124546 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT33744 NZ_AP021915:124349-124546 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3