Detailed information of TA system TA033731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3506850..3507687
• Replicon: chromosome
• Accession: NZ_AP021914
• Organism: Escherichia coli strain WP2-W18-CRE-03

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: H7Q98_RS16870
• Protein ID: WP_000227784.1
• Coordinates: 3507145..3507687 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: H7Q98_RS16865
• Protein ID: WP_001297137.1
• Coordinates: 3506850..3507161 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7Q98_RS16840	3501870..3502817	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
H7Q98_RS16845	3502839..3504830	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
H7Q98_RS16850	3504820..3505434	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
H7Q98_RS16855	3505434..3505763	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
H7Q98_RS16860	3505775..3506665	+	891	WP_000971336.1	heme o synthase	-
H7Q98_RS16865	3506850..3507161	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
H7Q98_RS16870	3507145..3507687	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
H7Q98_RS16875	3507743..3508678	-	936	WP_001368479.1	sel1 repeat family protein	-
H7Q98_RS16880	3509086..3510450	+	1365	WP_001000960.1	MFS transporter	-
H7Q98_RS16885	3510684..3511664	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
H7Q98_RS16890	3511857..3512348	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3510684..3511664	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T33731 WP_000227784.1 NZ_AP021914:3507145-3507687 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T33731 NZ_AP021914:3507145-3507687 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT33731 WP_001297137.1 NZ_AP021914:3506850-3507161 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT33731 NZ_AP021914:3506850-3507161 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3